The current study was conducted to investigate the role of alpha lipoic acid (ALA) against testicular toxicity-induced by lead acetate (PbAc) in rats. Four groups of adult Wistar albino rats (8 for each) were intubated daily for 56 days as follows; control (C)received dislled water; lead acetate at dose of 5mg/kg b.w (T1); ALA at dose of 60mg/kg b.w (T2) and and group T3 received both of PbAc + ALA at the same doses of above. Blood samples were collected at 0, 28 and 56 day of the experiment, then the sera were collected for determination of testosterone (T) and follicular stimulating hormone (FSH). At the end of the experiment, body weight, testes weight and epididymal sperm parameters was studied. Furthermore, histo-morphometric and histopathological study changes were examined. The results revealed a significant decrease in testes weight to body weight ratio, serum testosterone, sperm concentration and motility, diameters of seminiferous tubules, height of seminiferous epithelia and number of Leydig cells, moreover the results showed a significant increase in serum FSH, dead sperm and abnormal sperm morphology in group T1 when compared with the other groups. Comparing to lead acetate treated rats, group T3 showed an improvement at the level of the studied parameters, accompanied with mild congestion in the interstitial tissue with a marked developing proliferation of spermatogenic cells, as well as presence of mature spermatozoa in the seminiferous tubules. In conclusion, subchronic exposure of rats to lead acetate showed an amelioration of all reproductive parameters near to normal values due to the antioxidant effects of ALA and the histological changes of the testes confirmed such change in serum parameters and the beneficial role of ALA.

The study aimed to investigate the potential effect of resveratrol (RS) supplementation in attenuating kidney injury induced by hydrogen peroxide (H2O2) in male rats. Forty adult male rats were randomly assigned into four groups (10 each). The control group was orally supplemented with distilled water, and group G1 was orally supplemented with tap water containing 0.5% of H2O2, G2: was orally supplemented with tap water containing 0.5% of H2O2 and RS 87 mg/kg BW, and G3 was orally supplemented with RS 87 mg/kg BW. Blood samples were obtained before treatment and after 45 days of daily treatment. Serum concentrations of creatinine (Cr), urea nitrogen (UN), and total bilirubin were assessed. On day 45, male rats were euthanized, and kidney samples were taken to estimate malondialdehyde (MDA) and superoxide dismutase (SOD) concentrations, and other samples were obtained for histopathological examination. The results showed a significant increase in creatinine and BUN concentrations accompanied by a significant increase in MDA and a decrease in SOD in kidney tissue in G1 group male rats compared to other groups. Resveratrol partially restored the studied criteria related to renal functions by modulating the histopathological changes in the kidney induced by hydrogen peroxide. It could be concluded that supplementation of resveratrol to oxidatively stressed rats maintains the antioxidant defenses mechanism and could be helpful in the prevention of renal oxidative stress.

This present study was designed to investigate the effects of two levels of cadmium chloride (30 and 40 part per billion (ppb)) in drinking water on body weight, relative weight of testes and epididymus (head, body, tail), diameter and thickness of seminiferous tubules. Eighteen mature male rabbits were randomly divided into three equal groups; the control group (GI) were given ordinary tap water and animals in the first treated group (GII) were received 30 part per billion (ppb) of cadmium chloride in drinking water; while animals in the second treated group(GIII) were given 40 ppb of cadmium chloride in drinking water. Weight of animals were taken at the pre-treated period and every three weeks during the treated period. At the end of experiment the animals were killed, testis and epididymus were taken in order to measuring their weight. Samples testis were taken for histological study and for measuring the diameter of the seminiferous tubules and the thickness of the cells lining their. The results showed a significant increase (p<0.05) in the mean body weight of animals in the groups including the control (GI).The results also pointed to occurrence of a significant decrease (p<0.05) in the relative weight of testis and epididymus of both cadmium treated groups. Besides a significant decrease (p<0.05) in the mean of diameter the seminiferous tubules and the thickness of the cells lining them were occur after exposure to cadmium chloride. Concerning the histological study of the testis the results showed presence of acute necrotic changes modified by vaculation of cytoplasm of sertolli cells with decrease in number of spermatogenic cells, GIII group were more affected than GII group. On conclusion, cadmium chloride in drinking water (30 and 40 ppb) caused functional change in spermatogenesis in adult male rabbits.