Aims:The aim of this study is to evaluate the histopathological findings of healing phases in secondary wound defect on rabbit´s tongue, dressed with N-butyl -2-Cyanoacrylate (Histoacryl) tissue adhesive. Materials and Methods: Twenty domestic male rabbits had been selected. Two defects were done on each half of the tongue, 5mm oval shape, the right one left without covering, the left one covered by a thin layer of Histoacryl. Animals could be divided into four groups, each group contain five rabbits, according to sacrificing date into 3 days, 7 days, 14 days, and 30 days. Tongue specimen were collected after sacrificing the rabbit immediately, then divided into two halves, the right half was control one and the left half was the study one, giving off further two subdivisions for each group. The specimen were placed in formaldehyde and sent for histopathological examination regarding inflammatory and reepithelialization scoring. Results: The histopathological findings of this study revealed that there was a significant difference of inflammation between the two groups at 3 days, one week, one month, of tongue at p-value < 0.05, while there was no significant difference at two weeks period. In reepithelialization of the tongue a significant difference at 3 days at p-value < 0.05, and no significance at one week, two week, and one month. Conclusion: This study concluded that histoacryl tissue adhesive accelerate healing by decreasing inflammation, and inducing reepithelialization of mucosal secondary wound defects.