No abstract

To examine the effect of anti-inhibin and hCG-eCG treatments on reproductive hormonal profile in virgin cycling female rats, fifty four virgin cyclic female rats, aged 56 days and weighted 150-170 g., were randomly assigned to three equal groups (AI, eCG, and control). At late metaestrus and 54 h later, AI females were injected with anti-inhibin (1 µg/rat, ip) and normal saline (100µl/rat, ip), eCG females were injected with equine chorionic gonadotropin (20 IU/rat, ip) and human chorionic gonadotropin (10 IU/rat, ip), whereas control females were injected with normal saline (100µl/rat, ip). Eighteen females from each group were sacrificed after treatment (6 females/ group every 12 h for three times). Blood samples were obtained for hormonal assay (FSH, LH, estradiol 17B, prolactin, and ir-inhibin B). Serum FSH concentration of AI females increased significantly than other other groups throughout all periods. Also control females recorded significant elevation compared with eCG females. Serum LH concentrations registered no significant differences between AI and C groups, but they were significantly higher than eCG group only after 36 h. of treatment. Serum prolactin concentrations showed significant decline in AI group among experimental groups throughout all periods. Inhibin B levels, after 12, 24, and 36 h of treatment, decreased significantly in AI group compared with other groups. 17B-estradiol concentrations recorded insignificant differences among groups after 12 h. of treatment, while after 24 h., AI group level increased significantly than other groups. After 36 h. of treatment, AI and eCG groups recorded insignificant difference between each other, but, they were significantly higher than control. It can be concluded that anti-inhibin treatment increases the reproductive fecundity in female rats .

Resveratrol (RS) is widely used in medical fields as an antioxidant. Current research investigated ‎the protective role of RS supplement on histomorphometric and histopathological alterations in ‎testes were caused by hydrogen peroxide (H2O2) of rats as an animal model. Thirty-two adult ‎rats were utilized in this study, divided randomly into 4 equal groups as follows. The group C ‎was given tap water only and served as control, the 2nd group (G1) was given 0.5% of H2O2 of ‎tap water, the 3rd group (G2) was given tap water containing 0.5% of H2O2 and intubated RS ‎supplement at 87 mg/kg BW, and 4th group intubated RS supplement 87 mg/kg BW. after 56 ‎days of treatment, rats were euthanized, dissected then, specimens of testes tissue were collected ‎for histomorphometric and histopathological evaluation. Our results showed that administration ‎of H2O2 caused a significant histomorphometric with histopathological changes in the form of a ‎thickness of fibrous tunica albuginea, disarrangement of germ cells, necrosis of spermatogonia, ‎edema, and loss of sperms as compared to other groups. Meanwhile, these histological alterations ‎were partially attenuated in the G2 group that intubated resveratrol. Thus, the current study ‎concluded that resveratrol may have therapeutic value in the treatment of induced testicular injury ‎by H2O2 due to its antioxidant activity and attenuation of harmful effects of oxidative ‎stress through a mechanism that should be elucidated in future studies.

The current study was aimed to synthesized zinc oxide nanoparticles (ZnONPs) using aqueous extract of olive leaves (OLE), which is very simple and eco-friendly method. ZnONPs were formed by dissolving of OLE in zinc oxide solution with adjusted pH to 12. Zinc acetate dehydrate reduced to ZnONPs during mixing with OLE associated with change of the color solution from white to pale yellow color within a few minutes. The synthesized OLEZnONPs were separated by centrifugation (4000rpm/ 5min) , then characterized by Fourier Transmission Infrared Spectroscopy (FT-IR), X-ray diffraction (XRD) and Field emission-Scanning Electron Microscopy (FE-SEM) methods. The results of FT-IR showed that the functional group related to Zn-O at (433.98 to 416.67 cm_1), whereas X-RD at 2 theta diagnose the type of oxide formation as ZnO and determined particle size in range (20 – 30 nm). Besides, SEM image was showed the presence of hexagonal shape of ZnO nanoparticles (42.87nm). Therefore, the biogenic synthesis of zinc oxide nanoparticles using Olea Europaea leaves was simple, low coast, can be an alternative to chemical synthesis and the possibility of using in biomedicine field

This experiment was designed to evaluate the hepatoprotective effects of pomegranate seed oil against toxicity- induced by sodium fluoride and in normal rats. Twenty  adult female Wistar rats were divided into four equal group and treated daily for 40 days as follows: Group C administered  tap water and served as control , group T1 : received sodium fluoride 120ppm in drinking tap water, group T2: received both sodium fluoride 120ppm in drinking water and  administered orally pometone (pomegranate seed oil) 30mg/kg B.W. and group T3 : administered pomegranate seed oil as in group T2 orally. Fasting blood samples were collected at 0, 20 and 40 days to estimation of some biochemical parameters and oxidative stress biomarkers . In addition, sections from liver were taken at the end of the experiment for histopathological study. The results revealed that SF (group T1) caused a significant increase in serum aminotransferases (serum alanine aminotransferase and aspartate aminotransferase) activities, total cholesterol ,total bilirubin and peroxynitrite radical concentrations, while GSH concentration was a significantly decrease.  PSO caused an alleviation to the hepatic dysfunction induced by sodium fluoride (group T2) manifested through significant elevation of GSH concentration, in addition, a significant reduction in serum transaminases activity, total cholesterol, peroxynitrite radical and total bilirubin concentrations. In contrast, administration of pomegranate seed oil (group T3) showed no alterations in most of these parameters. Furthermore  histopathological examination of liver tissues of  group T1 manifested aggregation of mononucleated cells, proliferation of hepatocyte, cytoplasmic fat droplet and granulomatous lesion consists of aggregation of macrophage and lymphocyte. All these alteration in liver histology were modified by treatment of rats with pomegranate seed oil (group T2) and no pathological lesion was reported in group T3. On conclusion, this study documented the beneficial effect of pomegranate seed oil against the deleterious effects of SF on liver functions of adult female rats.