In this study, we report the cellular uptake studies of novel LX loaded nanoliposomes in H2O2 stress SH-SY5Y Cells synthesized by thin film evaporation method. We have isolated the smallest size nanoliposomes after 90 min ultrasonification, keeping Polydisperse Index as 0.259. The morphology, size, zepta potential and drug efficiency of prepared nanoliposomes are characterized by using Transmission Electron Microscope (TEM), particle size analyzer and High Pressure Liquid Chromatography (HPLC). The particle size analyzer have confirmed the particle size of nanoluposomes measured in range of 100-250 nm, whereas the shape of these nanoliposomes is almost spherical. The zeta potential of small size nanoliposomes was measured as -49.62 and encapsulation efficiency of the LX loaded nanoliposomes was 87%. The oxidative stress response in SH-SY5Y Cells for various doses of drug with and without nanoliposomes has affectively improved the cell-stress response up to 20% after 24 h of incubation at 37 degrees C. The results indicated that LX loaded nanoliposomes were taken by the cells effectively which ultimately improved the cell-stress response. Thus, this study confirmed that synthesized nanoliposomes are not only effective drug carriers but could be potentially used for delivery of genes, antibodies, and proteins in future.

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The surface of the olfactory epithelium of two species of cave loaches in China, Oreonectes polystigmus and O. guananensis, was studied by scanning electron microscopy (SEM). The structure of the olfactory organ of the two species is quite different from each other; as well, the distribution patterns of cell types in the olfactory epithelium of the studied species showed interesting features. A needle-like spine is visible on the lamellae of the olfactory organ of O. polystigmus, which is a unique character in this species. Ciliated non-sensory cells and microvillus sensory cells showed a different distribution in the two species, being more numerous in O. polystigmus. The non-sensory elements in O. guananensis were ciliated non-sensory cells, with microridges and chloride cells. The presence of chloride cells in the olfactory epithelium of O. guananensis may be of special interest in this species; otherwise, possible differences and physiological changes of the concerned species should be taken into account in order to explain such variability in cell types

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