New results obtained with microbore or microcapillary columns packed with DACH-DNB CSP (spherical silica, 5 pm) using conventional LC eluents or supercritical fluids are reported. Examples of direct chromatographic separations of complex mixtures of stereoisomers on: a) long, packed microbore or microcapillary columns and b) several columns coupled in series are shown; in the latter case, a home-made calculation procedure (Simulation for Multi-Column Chromatography -SMCC) was used to quickly select the optimal conditions (kind of stationary phases and relative columns length).
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