Here we describe a Sleeping Beauty (SB) transposition system that utilizes a conditional SB transposase allele, which can be activated by Cre recombinase to drive the transposition of a mutagenic transposon in virtually any tissue and control the type of cancer produced. To demonstrate the potential of this system for modeling cancer in mice, we used it to screen for hepatocellular carcinoma (HCC) associated genes in mice by specifically limiting SB transposition to the liver. Among 8,060 non-redundant insertions subsequently cloned from 68 tumor nodules we identified 19 highly significant candidate disease loci, which encode genes like EGFR and MET that are known HCC genes and others like UBE2H that are not strongly implicated in HCC but represent potential new therapeutic targets for treating this neoplasm. With these improvements, transposon-based insertional mutagenesis now offers great potential for better understanding the cancer genome and for identifying new targets for therapeutic development.

This paper reviews the key role that Earth Observations (EO) play in achieving the Sustainable Development Goals (SDGs) as articulated in the 2030 Agenda document and in monitoring, measuring, and reporting on progress towards the associated targets. This paper also highlights how the Group on Earth Observations (GEO) would contribute to ensure the actual use of EO in support of the 2030 Agenda; and how the Global Earth Observations System of Systems meets requirements for efficient investments in science and technology and a good return on investment, which is elaborated in the Addis Ababa Action Agenda on development financing. Through a number of examples, we first discuss how extensive EO use would: provide a substantial contribution to the achievements of the SDGs by enabling informed decision-making and by allowing monitoring of the expected results; improve national statistics for greater accuracy, by ensuring that the data are "spatially-explicit" and directly contribute to calculate the agreed SDG Targets and Indicators support the fostering of synergy between the SDGs and multilateral environmental agreements by addressing cross-cutting themes such as climate and energy; and facilitate countries' approaches for working across different development sectors, which is, according to the special adviser on the 2030 Agenda, a key challenge to achieve the SDGs. We then focus on the role that GEO could play in enabling actual use of EO in support of the 2030 Agenda by directly addressing the Strategic Development Goal 17 on partnerships.

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The effort and cost required to convert satellite Earth Observation (EO) data into meaningful geophysical variables has prevented the systematic analysis of all available observations. To overcome these problems, we utilise an integrated High Performance Computing and Data environment to rapidly process, restructure and analyse the Australian Landsat data archive. In this approach, the EO data are assigned to a common grid framework that spans the full geospatial and temporal extent of the observations -the EO Data Cube. This approach is pixel-based and incorporates geometric and spectral calibration and quality assurance of each Earth surface reflectance measurement. We demonstrate the utility of the approach with rapid time-series mapping of surface water across the entire Australian continent using 27 years of continuous, 25 m resolution observations. Our preliminary analysis of the Landsat archive shows how the EO Data Cube can effectively liberate high-resolution EO data from their complex sensor-specific data structures and revolutionise our ability to measure environmental change.ARTICLE HISTORY