Succinic dehydrogenase has usually been associated with a macromolecular intracellular unit (mitochondria) whenever the enzyme has been isolated. In an ultracentrifugal analysis of the composition of extracts of various organisms, Schachman et al. (1952) found that Rhodospirillum rubrum yielded particles with a wide range of sedimentation constants. Succinic dehydrogenase has been demonstrated in extracts of R. rubrum by Vernon and Kamen (1953) using the reduction of cytochrome c or a dye as the assay, and by Eisenberg (1953) with a manometric assay. This article describes the properties of a partially purified succinic dehydrogenase system from R. rubrum that is associated with particles that have the same sedimentation constants as do the particles bearing pigments. A soluble bacterial succinic dehydrogenase has been obtained from Azotobacter vinelandii (Repaske, 1954). MATERIALS AND METHODS Rhodospirillum rubrum strain S-1 was maintained as stab cultures in this medium: yeast extract, 0.2 per cent; peptone, 0.2 per cent; and agar, 2 per cent in distilled water. Mass cultures were grown in 500 ml glass-stoppered reagent bottles under constant incandescent illumination for 5 days at 25 C in the following medium: sodium lactate, 0.5 per cent; yeast extract, 0.5 per cent; peptone, 0.5 per cent; K2HPO4, 0.1 per cent; potassium citrate, 0.04 per cent; MgC12, 0.02 per cent; CaCl2, 0.002 per cent in distilled water adjusted to pH 7.2 with KOH. The cultures were harvested by centrifugation, washed twice, and stored at-20 C until used. Equal weights of alumina and cells were ground 1 Authorized for publication as Paper No. 1907 in the journal series of the Pennsylvania Agricultural Experiment Station on September 21, 1954.