Introduction Seasonal influenza is considered as one of the major causes of morbidity and mortality worldwide. This needs solutions to decrease burdens on the healthcare systems especially during the Coronavirus Disease (COVID-19) pandemic. Population knowledge, perception and attitude towards influenza vaccine during COVID-19 pandemic could have a positive impact to decrease mortality, morbidity and burdens on the healthcare system. This study focuses on investigating knowledge, attitude and practice (KAP) of Jordanian adults towards influenza vaccine during COVID-19 pandemic Methods This cross-sectional study recruited 1112 randomly selected Jordanian adults. A four-section questionnaire was designed and included questions about the demographic and clinical characteristics, perception about influenza, attitudes towards the role of influenza vaccine during COVID-19 pandemic and the factors that affect respondents’ practice towards influenza vaccine. Results 73.1% population were not vaccinated, and most of them were not willing to be vaccinated during COVID-19 pandemic. 55% of the population thought that influenza vaccine decreases the burdens on Jordanian healthcare system. Major refusal factor to be vaccinated was not considering influenza a threat (41.3%). People mostly got their information about influenza vaccine form the social media (64.3%) and there was neglected role of the pharmacist and physician. Conclusions The reinforcement of the role of pharmacists, physician and media to educate people about importance of influenza vaccine during COVID-19 is needed. Furthermore, campaigns should be conducted to increase the population awareness towards influenza vaccine uptake and its importance.