The introduction of nickel-titanium (NiTi) rotary instruments improved the root canal preparation of the narrow and curved root canals, especially after the introduction of thermomechanical treated (NiTi) alloys that have a high flexibility to prepare the curved canal reducing the common clinical complication, such as fracture, the change of the original shape of the root canal as a result of the change in the curvature of the curved root canal, the change of the working length which caused by the creation of the ledges, canal transporting and zipping especially in medium and highly curved canal. This study aimed to compare the shaping ability of two heat-treated nickel-titanium single file systems using reciprocation motion and rotary motion in curved canals. Materials and methods Thirty extracted human teeth with one curved root at 25 to 56 degrees were used, two NiTi single file systems were used to prepare the curved root canal in two groups: Reciproc R25 (Munich, Germany: VDW) group (n=15) and One Curve (Besancon, France: Micro-Mega) group (n=15). Curved root canal instrumentation outcomes were evaluated using cone-beam computed tomography (CBCT). Kruskal-Wallis with Bonferroni post hoc test was used to assess differences between working length, the angle and radius of curvature after instrumentation. Results There was a significant difference in angle and radius of curvature (-1.83° One Curve, -2.25° Reciproc blue and -0.18 mm One Curve, -0.19 mm Reciproc blue, respectively) (p<0.05) after instrumentation for both One Curve and Reciproc blue groups, and there was no significant difference in working length change (-0.16 mm One Curve, -0.32 mm Reciproc blue) after instrumentation of both One Curve and Reciproc blue groups (p>0.05). Conclusion The Reciproc blue single file system with reciprocation movement and One Curve with continuous movement cause a significant difference in curvature and radius of curved root canal affecting the original shape of the root canal with no significant difference in working length of the curved root canal.