The aim of this study was to examine the feasibility of combining an electro-Fenton pre-treatment and a biological treatment to remove azo dyes from aqueous effluents. The electro-Fenton reaction was performed in a two-compartment system involving a reference (saturated calomel electrode), a platinum anode and a graphite felt cathode. For the three considered dyes, high discoloration (in the range 77.1-97.8%) and low mineralization (in the range 7.7-18.8%) after 4-h reaction were in favor of the hybrid process, which was confirmed during subsequent experiments with methyl red sodium salt (MRSS) as a model dye. When potential-controlled electrolysis at 20.5 V/SCE was performed with a carbon-felt cathode surface of 378 cm 2 and a catalytic amount of ferric iron of 1 mmol L 21 , less than 1-h reaction was needed for an almost total discoloration (90.5%); while mineralization and oxidation remained low even after 48-h reaction, 11.1 and 57.2%, respectively. High discoloration level concomitantly to low mineralization and oxidation for MRSS removal which were in favor of a hybrid process, confirmed by the decrease of the COD on DOC ratio (45.1%) and the increase of the average oxidation state AOS (2.1). Final BOD 5 on COD ratio of 0.24 and an absence of toxicity of the treated solution (EC 50 5 165%) also showed the positive impact of an electro-Fenton pretreatment.

A High Performance liquid chromatographic method was developed for the determination of Cephalosporins like as: Cefuroxime axetil, Cefixime trihydrate, in pharmaceutical dosage forms. Isocratic separation was performed on an Enable C18 column (250 mm x 4.6 mm, 5.0µm) using Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265nm. The linearity was observed over a concentration range of 0.1-80 µg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2%, and the accuracy was > 99%. The developed method was used successfully for the determination of Cefuroxime axetil, Cefixime trihydrate, in Capsule, Tablet and dry syrup formulations.

Abstract.A new, simple and sensitive kinetic spectrophotometric method has been proposed for the determination of captopril (CPT) in pharmaceutical formulations. The method is based on oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) by ferric chloride followed by its coupling with the drug to form green-yellow coloured product with absorbance maximum at 395nm. The concentration of CPT was calculated using the calibration equation for the rate data and fixed time methods. The linearity range was found to be 0.5-22.5 µg mL -1 for each method. The correlation coefficients were 0.9994 and 0.9971 for rate data and fixed time methods respectively. The proposed methods were applied successfully for the determination of CPT in pharmaceutical formulations. Statistical comparison of the results shows that there is no significant difference between the proposed and official methods.

A new, simple and sensitive kinetic spectrophotometric method has been proposed for the determination of captopril (CPT) in pharmaceutical formulations. The method is based on oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) by ferric chloride followed by its coupling with the drug to form green-yellow colored product with absorbance maximum at 395nm. The concentration of CPT was calculated using the calibration equation for the rate data and fixed time methods. The linearity range was found to be 0.5–22.5 μg mL-1 for each method. The correlation coefficients were 0.9994 and 0.9971 for rate data and fixed time methods respectively. The proposed methods were applied successfully for the determination of CPT in pharmaceutical formulations. Statistical comparison of the results shows that there is no significant difference between the proposed and official methods.