Changing climates and associated increased variability pose risks to alfalfa (Medicago sativa L.) cultivation, with the requirement to establish, survive, and maintain production under water stress. Crop wild relatives (CWR) of alfalfa include populations that have evolved to survive in a number of different, extreme environments, but until recently have had limited use in breeding programs. Here we report on the phenotypic diversity of alfalfa crop wild relatives that were selected to represent extremes in drought tolerance (by sourcing germplasm from environments with extremes in low rainfall, high temperature, shallow soils, and winter freezing) with the aim of providing germplasm with drought tolerance and improved forage yield traits for breeding programs in both warm and cool dry temperate environments. Newly formed hybrids created between M. sativa, M. arborea L. (a woody shrub), and M. truncatula Gaertn. (an annual species from the Mediterranean region) were developed or acquired to introduce new genetic diversity from the tertiary genepool. Preliminary characterization and evaluation was used for taxonomic classification, and to identify wild accessions and pre‐bred (hybrid) lines that offer new diversity for growth habit, seed size, fall dormancy, and forage yield. The accessions and pre‐breeding lines described have been donated to the Australian Pastures Genebank for conservation and distribution.

Journal of Agricultural Sciencewww.agrivita.ub.ac.id up to 300 kg/ha of nitrogen, which improves soil fertility and increases the yield of subsequent crops.All alfalfa varieties cultivated in Kazakhstan belong to two tetraploid species of M. sativa L. and M. varia Mart. Varieties of M. sativa L. are cultivated in the southern, southeastern, and western parts of Kazakhstan, and varieties of M. varia Mart. -in the northern part of the republic due to their increased winter hardiness. In the natural flora of Kazakhstan, there are seven species of perennial alfalfa of the Falcago (Reichb) Grossh subgenus of 21 species as per the latest classification compiled by P. A. Lubenets. The diploid species are M. difalcata Sinsk.,

In Kazakhstan, fungal diseases root rot and Ascochyta strongly affect the growth and yield of peas. Traditional breeding practices have resulted in improved resistance to these diseases in adapted varieties and lines, which is the main way to minimize yield losses. The global problem for breeders is to identify genes for disease resistance and use them in breeding programs. At the moment, promising, highly resistant to fungal diseases have been identified. In this work, modern methods of experiment, observation and accounting, selection and preparation of land plots, laying and experimenting with physiological and biological parameters of pea samples were used. Monitoring was carried out to determine the phytopathological and immunological evaluation of 59 varieties and lines of peas to fungal diseases was carried out. As a result of the analysis, 16 genotypes were highly resistant (I), 32 resistant (R) and 11 moderately resistant (MR) to Ascochyta in the natural background of the disease. At the same time, 21 genotypes were highly resistant (I), 26 resistant (R), 5 moderately resistant (MR), 6 moderately susceptible (MS) and 1 susceptible (S) to root rot on a natural background. On an artificial infectious background, the result showed that 9 genotypes were highly resistant (I), 43 resistant (R), and 7 moderately resistant to Ascochyta. And 19 genotypes were highly resistant (I), 33 resistant (R), 3 moderately resistant (MR), 2 moderately susceptible (MS) and 2 susceptible (S) to root rot. As a result of phenological analysis, in most pea samples, ascochitosis disease and root rot were pronounced, but with the least damage. The vegetation period was 112 days in 2021, and up to 93 days in 2022. According to the analysis indicators, all pea samples are very useful for identifying donors and can be included in breeding programs for hybridization to create new productive and resistant varieties.