An estimation and comparison of chlorophyll content and photochemical yield of photosystem II obtained from solar and artificially induced chlorophyll fluorescence is presented here, along with a new technique to estimate photochemical yield from the solar-induced method.

Abstract:A comprehensive evaluation of the recently developed Fraunhofer line depth (FLD) and laser-induced saturation pulse (FLD-LISP) method was conducted to measure chlorophyll fluorescence (ChlF) parameters of the quantum yield of photosystem II (Φ PSII ), non-photochemical quenching (NPQ), and the photosystem II-based electron transport rate (ETR) in three plant species including paprika (C3 plant), maize (C4 plant), and pachira (C3 plant). First, the relationships between photosynthetic photon flux density (PPFD) and ChlF parameters retrieved using FLD-LISP and the pulse amplitude-modulated (PAM) methods were analyzed for all three species. Then the relationships between ChlF parameters measured using FLD-LISP and PAM were evaluated for the plants in different growth stages of leaves from mature to aging conditions. The relationships of ChlF parameters/PPFD were similar in both FLD-LISP and PAM methods in all plant species. Φ PSII showed a linear relationship with PPFD in all three species whereas NPQ was found to be linearly related to PPFD in paprika and maize, but not for pachira. The ETR/PPFD relationship was nonlinear with increasing values observed for PPFDs lower than about 800 µmol m −2 s −1 for paprika, lower than about 1200 µmol m −2 s −1 for maize, and lower than about 800 µmol m −2 s −1 for pachira. The Φ PSII , NPQ, and ETR of both the FLD-LISP and PAM methods were very well correlated (R 2 = 0.89, RMSE = 0.05), (R 2 = 0.86, RMSE = 0.44), and (R 2 = 0.88, RMSE = 24.69), respectively, for all plants. Therefore, the FLD-LISP method can be recommended as a robust technique for the estimation of ChlF parameters.Keywords: C3 plant; C4 plant; Fraunhofer line depth and laser-induced saturation pulse (FLD-LISP) method; non-photochemical quenching (NPQ); photochemical yield of photosystem II (Φ PSII ); photosystem II-based electron transport rate (ETR); solar-induced chlorophyll fluorescence (SIF)

Evapotranspiration (ET) plays an important role in the study of regional long-term water cycles. The water cycle in Mongolia has been seriously affected by global warming and the intensification of human activities. A significant relationship exists between climate factors and ET. In this paper, the temporal and spatial fluctuations and stability of ET in Mongolia from 2001 to 2020 were studied by using MOD16A2 ET, MOD13A2 NDVI and the climate data of ERA5-Land. ET trends were analysed by using the Breaks for Additive Season and Trend (BFAST) software package, Theil–Sen median trend analysis, Mann–Kendall method and Hurst index. The correlations between ET and temperature (Tem), precipitation (Pre), net solar radiation (Nsr), soil moisture (Swl) and human activities were determined by partial correlation analysis and a geographic detector. In the past 20 years, ET increased significantly in 49.4% of Mongolia, and NDVI also showed a significant increasing trend. BFAST detected two mutation years. ET decreased rapidly from 2006 to 2007 and increased rapidly from 2015 to 2016. In addition to winter, the meteorological factor that had a significant positive impact on ET in the east and west was Pre, whereas the impact of Tem was more obvious in central Mongolia. In winter, Tem had a great impact on ET. In the vegetation growing season, the joint action of NDVI and Pre greatly positively contributed to ET. The geographical detector showed that the influence of annual human factors on ET was weakened by changes in NDVI and Pre. In the growing season, Tem and Nsr increased nonlinearly to ET, and other natural and human factors showed bivariate enhancement. These results will help to understand the responses of ET changes to natural factors and human activities in Mongolia and provide data support for future research on ET and the water cycle.

The phenological parameters estimated from different data may vary, especially in response to climatic factors. Therefore, we estimated the start of the growing season (SOS) and the end of the growing season (EOS) based on sunlight-induced chlorophyll fluorescence (SIF), the normalized difference vegetation index (NDVI) and the near-infrared reflectance of vegetation (NIRv). The SIF, NDVI and NIRv breakpoints were detected, and the trends and change-points of phenological parameters based on these data were analyzed. The correlations between the phenological parameters and snow-related factors, precipitation, temperature, soil moisture and population density were also analyzed. The results showed that SIF and NIRv could identify breakpoints early. SIF could estimate the latest SOS and the earliest EOS. NDVI could estimate the earliest SOS and the latest EOS. The change-points of SOSSIF were mostly concentrated from 2001 to 2003, and those of SOSNDVI and SOSNIRv occurred later. The change-points of EOSSIF and EOSNIRv were mostly concentrated from 2001 to 2007, and those of EOSSIF occurred later. Differently from the weak correlation with SOSSIF, SOSNDVI and SOSNIRv were significantly correlated with snow-related factors. The correlation between the meteorological factors in the summer and autumn and EOSSIF was the most significant. The population density showed the highest degree of interpretation for SOSNIRv and EOSNDVI. The results reveal the differences and potentials of different remote-sensing parameters in estimating phenological indicators, which is helpful for better understanding the dynamic changes in phenology and the response to changes in various influencing factors.

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