Detecting the amplification and expression of human epidermal growth factor receptor (HER2) is important for planning trastuzumab treatment for patients with gastric carcinoma. The present study aimed to analyse HER2 amplification and expression in primary gastric adenocarcinoma tumours and metastatic lymph nodes using microarray methods, and to assess the potential contribution of these methods to treatment planning. In total, 60 patients with lymph node metastasis were included in the present study. Microarray blocks were obtained from the tissue blocks of primary tumours and metastatic lymph nodes. HER2 expression and amplification were investigated using immunohistochemical and silver in situ hybridisation (SISH) methods, respectively. Following immunohistochemical evaluation of HER2 in primary tumours, the sensitivity and specificity of the microarray method relative to the single block method were 69 and 100%, respectively. For HER2 detection in microarray block sections from primary tumours, the sensitivity and specificity of the SISH method relative to immunohistochemistry were 56 and 100%, respectively. When using SISH in microarray blocked sections, there was a high degree of concordance (98% concordance rate) between HER2 amplification in the primary tumour and the metastatic lymph node. Furthermore, the sensitivity and specificity of metastatic lymph node results relative to those of the primary tumour were 100 and 98%, respectively. Overall, the single block method was more reliable compared with the microarray method for planning treatment. When microarray blocking was used, a large number of samples must be tested to ensure reliable results. The immunohistochemical method is recommended as the first step as SISH alone increases the risk of false-negative results. Assessing HER2 amplification for treatment planning would be beneficial for primary tumours, as well as metastatic lymph nodes.

Background / Aims: Most stomach adenocarcinomas are still recognized in the advanced stages despite improvements in diagnostic methods. The aim of this study is investigate immunohistochemistry of mucin core proteins (MUCs) and Sonic Hedgehog (SHH) antibody expressions in gastric adenocarcinomas in relation to prognosis and Human epidermal growth factor receptor-2 (HER-2) levels. Material and Methods: Pathologic diagnoses were reclassified according to World Health Organization clasification. MUC1, MUC2, MUC5AC, MUC6, SHH and HER-2 antibodies examination was performed immunohistochemically. Tissue microarray technique was used in the study. Results: HER2 overexpression was associated with luminal staining with MUC1. When HER2 expression and SHH expressions were compared, all cases with HER2 overexpression were found to be positive with SHH. This is the first study to determine the relationship between SHH, MUC1 and HER2 immunohistochemical expressions in gastric adenocarcinomas. Conclusion: Examining the HER2 relationship between SHH and MUC1 expressions we have shown, with future genetic and molecular studies, will provide an understanding of different malignancy pathways in gastric adenocarcinomas. We think that the treatment rate in stomach cancer will be increased with agents aimed at preventing these pathways. Key words: Gastric adenocarcinoma; mucins; Sonic Hedgehog; HER2.