No abstract

No abstract

Pemalsuan daging sapi yang dicampur dengan daging babi merupakan salah-satu permasalahan umat Muslim yang sering ditemukan di Indonesia. Penelitian ini bertujuan untuk mendeteksi daging babi pada daging sapi segar dan masak menggunakan marker genetik DNA mitokondria gen Cytochrome b (mt-DNA Cyt b) dengan duplex-PCR. Penelitian ini menggunakan 6 sampel daging segar dan 6 sampel daging yang dimasak. Sampel daging babi diperoleh dari Rumah Potong Babi (RPB), sedangkan sampel daging sapi dibeli dari supermarket di Kota Surakarta. Sampel daging masak disiapkan dengan cara merebus daging pada suhu 100 o C selama 30 menit. Kontaminasi daging babi pada daging sapi segar maupun masak dirancang sebesar 0, 1, 5, 10, dan 25%. Selanjutnya, total DNA diisolasi dari sampel tersebut dan polymerase chain reaction (PCR) dilakukan menggunakan primer spesifik untuk mengamflifikasi fragmen spesifik masing-masing spesies. Hasil penelitian menunjukkan bahwa total DNA berhasil diekstraksi dari sampel daging sapi, daging babi, dan campuran keduanya baik pada sampel daging segar maupun masak. Selanjutnya, elektroforesis pada agarosa 1,5% menunjukkan bahwa duplex-PCR mt-DNA Cyt b berhasil mendeteksi kontaminasi daging babi pada daging sapi segar maupun masak hingga level 1%. Keberadaan daging babi pada sampel ditunjukkan dengan adanya pita DNA sebesar 398 bp. Kesimpulan penelitian ini adalah duplex-PCR mt-DNA Cyt b sangat sensitif dalam mendeteksi cemaran daging babi pada daging sapi segar maupun masak.

No abstract

The aim of this study was to compare the utilization of decomposer from bioethanol waste (DLB) with various commercial decomposers on quality of compost made from cattle dung. This study used completely randomized design (one-way ANOVA) with four treatments and five replications. Materials used in this study were cattle dung, water, molasses, DLB and various commercial decomposers (A, B, and C). The treatments in this study were cattle dung + BLB 0.5% (P0), cattle dung + decomposer A 0.5% (P1), cattle dung + decomposer B 0.5% (P2) and cattle dung + decomposer C 0.5% (P3). Then, the mixtures were aerobically ripened until four weeks. During composting, compost was stirred weekly. Furthermore, mature compost was analyzed to evaluate the amount of C Organic, total N, total P, total K, total Ca, total Mg, C/N ratio and pH value. The analysis of variance showed that there was no difference of the quality and pH value of compost made from cattle dung with utilization of DLB and commercial decomposers. It could be concluded that utilization of DLB in composting of cattle dung had identically quality and pH value of compost with utilization of commercial decomposers, therefore, this technology could be an alternative to process waste from home industry of bioethanol.