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A ten months investigation was conducted to know the status of bacterial flora in the farmed hybrid catfish (Clarias hybrid) and respective pond water from the fish pond of a commercial fish farm of Bangladesh. The average total load in pond water, slime and kidney of fish varied from 2.1×10 3 to 4.1×10 6 CFU/ml of water, 5.6×10 3 to 4.4×10 9 CFU/g of slime and 1.8×10 1 to 2.7×10 4 CFU/g of kidney. Coryneforms and Flavobacterium were the dominant bacteria in the observed fish-organs and pond-water. Higher bacterial load was recorded in the months of September and October. Sensitivity of the isolated Aeromonads and Pseudomonads to various antibacterial agents was determined. Higher percentage of resistant Aeromonads and Pseudomonads were observed against oxytetracycline followed by erythromycin. On the contrary, the percentage of resistant Aeromonads and Pseudomonads was lower against oxolinic acid.

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An invesigation on optimal temperature for the growth and protease production of a mesophilic bacterium, Aeromonas hydrophila was carried out. The studied 12 strains of fish origin were collected and stored at-80•Ž.