Background:To elucidate molecular signatures of chronic periodontitis (CP) using gingival tissue samples through omics-based whole-genome transcriptomic and whole protein profiling.Methods: Gingival tissues from 18 CP and 25 controls were analyzed using gene expression microarrays to identify gene expression patterns and the proteins isolated from these samples were subjected to comparative proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The data from transcriptomics and proteomics were integrated to reveal common shared genes and proteins. Results:The most upregulated genes in CP compared with controls were found as MZB1, BMS1P20, IGLL1/IGLL5, TNFRSF17, ALDH1A1, KIAA0125, MMP7, PRL, MGC16025, ADAM11, and the most upregulated proteins in CP compared with controls were BPI, ITGAM, CAP37, PCM1, MMP-9, MZB1, UGTT1, PLG, RAB1B, HSP90B1. Functions of the identified genes were involved cell death/survival, DNA replication, recombination/repair, gene expression, organismal development, cell-tocell signaling/interaction, cellular development, cellular growth/proliferation, cellular assembly/organization, cellular function/maintenance, cellular movement, B-cell development, and identified proteins were involved in protein folding, response to stress, single-organism catabolic process, regulation of peptidase activity, and negative regulation of cell death. The integration and validation analysis of the transcriptomics and proteomics data revealed two common shared genes and proteins, MZB1 and ECH1. Conclusion:Integrative data from transcriptomics and proteomics revealed MZB1 as a potent candidate for chronic periodontitis.

Smoking negatively affects the prognosis of periodontal disease by impairing tissue healing. While micronucleus is the most popular parameter for demonstrating DNA damage, inflammatory cell and vascular densities are the most evaluated parameters for determining histopathologic changes in the periodontium. This study aimed to study the effects of periodontitis and cigarette smoking on genotoxic changes in exfoliated oral epithelial cells and histopathologic changes in periodontal tissue. A crosssectional study was conducted between November 2018 and July 2019 at a dental university hospital in Turkey, and registered as NCT05484765. Eighty systemically healthy subjects were divided into four groups according to periodontal status and smoking habits: 20 smokers with generalized periodontitis (SGP), 20 nonsmokers with generalized periodontitis (NGP), 20 smokers with healthy periodontium (SHP), and 20 nonsmokers with healthy periodontium (NHP). For each study participant, full-mouth clinical periodontal parameters (CPPs) were measured, smear samples were taken from buccal and gingival mucosa, and periodontal tissue was biopsied from the maxillary molars. Cytogenetic and histopathologic assays (primary and secondary outcomes) were conducted using Feulgen reaction and hematoxylin-eosin staining, respectively. The mean CPPs of healthy periodontium groups were lower than generalized periodontitis groups. No significant differences were found between other groups regarding CPPs. Buccal micronuclei counts in groups decreased with the highest to lowest counts occurring in the order SGP > SHP > NGP > NHP. Gingival micronuclei counts in groups decreased from SGP > SHP > NGP = NHP. The most intense inflammatory cell and vascular densities occurred in SGP and NGP groups, respectively; and the mildest values were in healthy periodontium groups. Histopathological damage score decreased significantly by group in order SGP > NGP > SHP > NHP. The synergy arising from the combination of smoking and periodontitis exposures exacerbates genotoxic and histopathologic damage in oral cells and the periodontium.Abbreviations: BOP = bleeding on probing, CPPs = full-mouth clinical periodontal parameters, NGP = nonsmokers with generalized periodontitis, NHP = nonsmokers with clinically healthy periodontium, PD = probing depth, SGP = smokers with generalized periodontitis, SHP = smokers with clinically healthy periodontium.

BackgroundOur previous study explored the molecular signatures of generalized aggressive periodontitis (GAgP) using gingival tissues through omics‐based‐whole‐genome transcriptomic analysis. This continuation study aimed to investigate the whole protein profiling of these gingival samples through liquid chromatography‐mass spectroscopy/mass spectroscopy (LC‐MS/MS) analysis and to validate the identified proteins through immunohistochemistry to provide further evidence for the quality of the results.MethodsIn previous study, gene expression patterns were identified in gingival tissues from 23 GAgP and 25 control individuals. In the current study, comparative proteomic analysis was performed on isolated proteins from the same study groups using LC‐MS/MS analysis. The data from the transcriptomics study published before and the proteomics data were integrated to reveal any common genes and proteins. Additionally, immunohistochemical analysis was conducted to further investigate the findings.ResultsThe most upregulated proteins in patients compared to controls were ITGAM, AZU1, MMP9, BPI, UGGG1, MZB1, TRFL, PDIA6, PRDX4, and PLG. The top six pathways associated with these proteins were involved in innate immune system, post‐translational protein phosphorylation, interleukin‐4 and ‐13 signaling, toll‐like receptors cascades, and extracellular matrix organization. Based on the integration and validation analysis of transcriptomics and proteomics data, as well as immunohistochemical analysis, MZB1 was identified as a shared gene and protein that were upregulated in the patients.ConclusionsMZB1 is a protein that is involved in the development of B cells and the production of antibodies. Its upregulation in periodontitis suggests that there may be a dysregulation of the immune response in this condition, and MZB1 may be a potent biomarker for periodontitis.

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This double-blinded and split-mouth design, randomized feasibility study aimed to assess the efficacy of prophylactic and therapeutic use of a new age NitrAdineTM-based brushing solution (PerioTabs®) on the clinical parameters before and after periodontal therapy. Four subjects were randomly assigned to two treatment groups according to periodontal infection: PerioTabs® and placebo. At the first appointment, a split-mouth scaling was performed in all participants. All oral care instructions concerning the use of effervescent tablets were explained to the participants in detail. The morning after the tablets were finished, a full-mouth scaling was completed in all participants. All parameters were recorded at 0, 11, and 40 days. According to the results, both the therapeutic and prophylactic interventions showed similar impacts on the gingival index and probing depth compared to the placebo in all patients. The bleeding on probing was further reduced at the PerioTabs® site at baseline until day 40 compared to the placebo in the gingivitis patients with both interventions; at both 11 and 40 days in the periodontitis patients receiving the therapeutic intervention; and at baseline to day 40 in the periodontitis group receiving the prophylactic intervention., While the preliminary results of this new-age brushing solution appear to be a promising approach for a future therapy of periodontal diseases, further research on a larger sample size is needed to draw firm conclusions.