The essential oil from aerial part of Marrubium deserti De Noé (Lamiaceae), obtained by hydrodistillation was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), and evaluated for in vitro antimicrobial activity. The antioxidant activity was determined using three in vitro assays: scavenging effect on DPPH, the ABTS test and the phosphomolybdenum method. Thirty-seven compounds were identified in the oil, with germacrene D as the major component (45.7%). This oil was characterized by an important hydrocarbon fraction (78.1%) and by the predominance of sesquiterpenes (67.4%). M. deserti essential oil had no activity on the tested microorganisms (Staphylococcus aureus ATCC 25923, Echerichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans and Aspergillus flavus). However the oil presented an antioxidant activity.
Marrubium vulgare, plant species belonging to Marrubium genus, is widespread in the Mediterranean areas, introduced elsewhere and also cultivated in many countries. Its oil is recognized to possess a considerable biological activities with varied chemical composition. This paper aims to overview the chemical composition and biological activities of M. vulgare essential oil's considered as a medicinal plant, widely used in folk medicine overall the world. In essential oils of M. vulgare, germacrene D, β-caryophyllene, β-bisabolene, bicyclogermacrene and carvacrol are generally considered as either mains or minor constituents and each species presents its own composition. Sesquiterpenoids were the dominant fraction while monoterpenoids were present in appreciable or in trace amount. Oxygenated fractions dominated in monoterpenes however, hydrocarbon fraction overpowered in sesquiterpenes. These oils are biologically active, they exhibit an antioxidant and antimicrobial activities and other activities. Due to the variability of composition of essential oil, further studies are necessary, particularly regarding their chemical's which may cause an important change in the biological activities of oils and probably defined different chemotype.
The chemical composition and nitrogen distribution of milk from two ewe's breed (Ouled-Djellal and Rumbi) (n = 20 each) reared in Algerian area steppe, were studied. The ewes were balanced for age and weight. All were in middle period of lactation. Individual milk samples were taken from each ewe third time from lactating period during spring season. Rumbi ewe's milk gave the highest values (p≤0.01) for lactose (4.89±0.61%), solid non-fat (11.19±0.87%) and density (1037.57±3.78) than Ouled-Djellal ewes (4.38±0.45%, 10.24±0.77% 1033.82±2.59, respectively) and lowest values (p≤0.01) for freezing point (-0.57±0.05°C vs -0.53±0.02°C). No significant difference was observed between the two breeds on fat, protein, total solids. The mean percentages were 6.26±1.38%, 5.54±0.76 and 16.51±1.44%, respectively for Ouled-Djellal ewes, while those of Rumbi's were 5.66±3.52%, 5.91±1.10% and 16.85±3.32%, respectively. No statistical differences related to the breed were found in the milk for all nitrogen fractions. Rumbi ewe's milk contains about 1.01% nitrogen however Ouled-Djellal's hold about 0.96%. Total nitrogen content is distributed between non-protein nitrogen (0.09% in all breeds) and protein nitrogen (0.93 vs 0.87% for Rumbi and Ouled -Djellal ewes respectively). The protein nitrogen includes casein nitrogen (0.76 vs 0.71) and whey protein nitrogen (0.20 vs 0.19%) from Rumbi and Ouled-Djellal ewes, respectively. Algerian sheep breeds were not actually selected for their milk production; selection program should be implemented to improve milk production and increase fat and protein contents. Thus further studies should carry out on milk ability and milk yield of these breeds.
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