Lentil landraces have been on-farm conserved in Morocco for home-consumption and for their adaptation potential to specific environment constraints. Landraces might constitute sources of optional genes of interest linked to environmental stresses resilience add to nutritional quality traits valuable for lentil crop genetic improvement. Thus, the current study aims to assess the available genetic diversity among eight lentil landraces collected from the main lentil cropped areas in Morocco by using SDS-PAGE and determinate their nutritional value compared to four improved varieties. Results evidenced the genetic richness of Moroccan's landraces according to the range of polymorphism rate (17.5%-80%), allelic richness (1.100-1.775), expected heterozygosity(0.044-0.334) and Shannon-Weaver's genetic diversity Index (0.072-0.482). In addition, a large range of nutrients content variability was recorded in regards to crude proteins (26.8-33.8%), total carbohydrate (46.4-58.3%), neutral fibers (39.7-46.4%), lipids content (1.13-2.10 mg/100g) composed mostly of unsaturated fatty acid (83.9%) revealing the healthy source of lentils. The major compounds of total phenols (7.60 mg/100g) are the condensed tannins (6.04mg/g DM) which concentrated mainly in seed coat (67%). The large range variability of micronutrients concentration as iron (6.57-8.26), magnesium(102.0-126.8), zinc (3.74-4.83), calcium (44.8-55.8), phosphorus(431.4-454.1), potassium (922.0-956.5) and manganese (1.27-1.37) revealed the sustainability of local genetic cores in providing rich and healthy nutrient food with high potential in developing new varieties resilient to climate change.