Scent marking and over-marking are important forms of communication between the sexes for many terrestrial mammals. Over the course of three experiments, we determined whether the amount of time individuals investigate the scent marks of opposite-sex conspecifics is affected by four days of olfactory experience with those conspecifics. In experiment 1, female meadow voles, Microtus pennsylvanicus, spent more time investigating the scent mark of the novel male conspecific than that of the familiar male donor, whereas male voles spent similar amounts of time investigating the scent mark of the familiar female and a novel female conspecific. In experiment 2, voles were exposed to a mixed-sex over-mark in which subjects did not have four days of olfactory experience with either the top-scent donor or the bottom-scent donor. During the test phase, male and female voles spent more time investigating the scent mark of the opposite-sex conspecific that provided the top-scent mark than that of a novel, opposite-sex conspecific. Male and female voles spent similar amounts of time investigating the scent mark of the bottom-scent donor and that of a novel opposite-sex conspecific. In experiment 3, voles were exposed to a mixed-sex over-mark that contained the scent mark of an opposite-sex conspecific with which they had four days of olfactory experience. During the test phase, male voles spent more time investigating the mark of the familiar, top-scent female than the scent mark of a novel female donor but spent similar amounts of time investigating the mark of the familiar, bottom-scent female and that of a novel female donor. In contrast, female voles spent more time investigating the mark of a novel male donor than that of either the familiar, top-scent male or that of the familiar, bottom-scent male. The sex differences in the responses of voles to scent marks and mixed-sex over-marks are discussed in relation to the natural history and non-monogamous mating system of meadow voles.

Previous studies have shown that individuals responded preferentially to the mark of the top-scent donor relative to that of the bottom-scent donor of an over-mark. However, terrestrial mammals are likely to encounter over-marks consisting of the scent marks of more than two same-sex conspecifics in the intersections of runways, near the nests of sexually receptive female conspecifics, and inside and along the borders of the territories of conspecifics. We determined how meadow voles, Microtus pennsylvanicus, respond to the marks of the top-, middle-, and bottom-scent donors of an over-mark. We tested the hypothesis that voles exposed to an over-mark will respond preferentially to the scent marks that were deposited more recently, the scent marks that were on top or near the top of the over-mark, compared to the scent marks that were deposited earlier or near the bottom of the over-mark. Voles spent more time investigating the mark of the top-scent donor than that of the either the middle- or bottom-scent donor. However, males but not female voles spent more time investigating the middle-scent mark than the bottom-scent mark. We also tested the hypothesis that voles evaluate and respond to over-marks differently from single scent marks. Voles spent more time investigating the marks of the top-, middle-, and bottom-scent donors compared to scent marks that were not part of the over-mark. Voles can distinguish among the overlapping scent marks of three scent donors and sex differences exist in the values they appear to attach to each of these scent marks.

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