Streptococcus uberis is one of the most common pathogens of clinical mastitis in the dairy industry. Knowledge of pathogen transmission route is essential for the selection of the most suitable intervention. Here we show that spectral profiles acquired from clinical isolates using matrix-assisted laser desorption ionization/time of flight (MALDI-TOF) can be used to implement diagnostic classifiers based on machine learning for the successful discrimination of environmental and contagious S. uberis strains. Classifiers dedicated to individual farms achieved up to 97.81% accuracy at cross-validation when using a genetic algorithm, with Cohen’s kappa coefficient of 0.94. This indicates the potential of the proposed methodology to successfully support screening at the herd level. A global classifier developed on merged data from 19 farms achieved 95.88% accuracy at cross-validation (kappa 0.93) and 70.67% accuracy at external validation (kappa 0.34), using data from another 10 farms left as holdout. This indicates that more work is needed to develop a screening solution successful at the population level. Significant MALDI-TOF spectral peaks were extracted from the trained classifiers. The peaks were found to correspond to bacteriocin and ribosomal proteins, suggesting that immunity, growth and competition over nutrients may be correlated to the different transmission routes.

One of the leading scholars and academic administrators of his time, Benjamin Jowett (1817–1893) was Master of Balliol College as well as Regius Professor of Greek and, for a time, vice-chancellor at Oxford University. Along with his achievements in the area of academic reform, Jowett is remembered for this four-volume translation of Plato's dialogues. Characterising Plato as the 'father of idealism', Jowett reminds readers that while 'he may be illustrated by the writings of moderns … he must be interpreted by his own, and by his place in the history of philosophy'. In this first volume, he includes fourteen early and middle dialogues such as Charmides, Apology, Crito, and Phaedo, each presented with a separate editorial introduction. Jowett's work represents a towering achievement in the field of classical and philosophical studies that had important influence on the subsequent study of Plato.

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