Mutants defective in polyol metabolism and/or in protoperithecial development were selected in Neurospora tetrasperma, a species in which protoperithecial development occurs at nonpermissively high temperature if certain polyols are used in lieu of sucrose as carbon source. Mutants selected for nonutilization of one of the four polyols tested, glycerol, mannitol, sorbitol, or xylitol, were usually found to be nonutilizers of the other three polyols as well. Mutants blocked at various stages of protoperithecial development complemented pairwise to produce more advanced developmental stages, usually mature protoperithecia and, when of opposite mating type, mature perithecia. About one-third of the mutants manifested both polyol auxotrophy and defective protoperithecial development upon initial isolation, but protoperithecial defectiveness in such mutants usually showed erratic segregation in crosses and/or instability to repeated vegetative transfer, whereas polyol auxotrophy usually did not and was, therefore, studied further. Two glycerol nonutilizing strains were introgressed into N. crassa to facilitate genetic analysis. One, glp-4, lacked both inducible and constitutive glycerol kinase and mapped to linkage group VI, between ad-1 and rib-1; the other, glp-5, lacked glyceraldehyde kinase and mapped to linkage group I, proximal to ad-9. Another mutant, gly-u(234), has been reported by other investigators to lack inducible glycerol kinase but to map to linkage group I, distal to ad-9.

Before-after-control-impact (BACI) experimental designs are commonly used in large-scale experiments to test for environmental impacts. However, high natural variability of environmental conditions and populations, and low replication in both treatment and control areas in time and space hampers detection of responses. We compare the power of two asymmetric BACI (aBACI) designs to two staircase designs for detecting changes in juvenile steelhead (Oncorhynchus mykiss) abundance associated with a watershed-scale stream restoration experiment. We performed a simulation study to estimate the effect of a 25% increase in steelhead abundance using spatial and temporal estimates of variance from an ongoing study, and determined the power of each design. Experimental designs were then applied to three streams and each stream was composed of three 4 km long sections. We compared the power of a single treatment section in one stream (BACI-1), three simultaneous treatments of all sections in one stream (BACI-3), three sequential treatments in one stream (STAIRCASE-1), and three sequential treatments in one section in each stream (STAIRCASE-3). All designs had ≥ 94% power to detect a 25% increase in abundance assuming average variance. Under worst-case variance (i.e., upper 95% confidence limits of historical variance estimates), the STAIRCASE-3 design outperformed the BACI-1, BACI-3, and STAIRCASE-1 designs (i.e., 77%, 41%, 8%, and 33% power respectively). All the designs estimated the effect of the simulated 25% abundance increase, but the length of the confidence interval was much shorter for the STAIRCASE-3 design compared to the other designs, which had confidence intervals 58-596% longer. The STAIRCASE-3 design continued to have high power (88%) to detect a 10% change in abundance, but the power of the other designs was much lower (range 34-56%). Our study demonstrates that staircase designs can have significant advantages over BACI designs and therefore should be more widely used for testing environmental impacts.