In order to obtain high production rates of sawn timber, the sawmilling industry can use laser scanning, allowing knot detection and machine strength grading. In particular, laser scanners measure grain angle using the so-called tracheid effect on wood surface where an elliptic scattering of the laser light can be observed. This paper aims to describe the light scattering obtained by a laser beam projection on wood surface and to assess the accuracy of such fiber orientation measurement on five European species. Firstly, fiber orientation measurement error was assessed by rotating samples. Secondly, the description of the scattering effect was done considering ellipse axis ratios and areas. This was studied according to several parameters such as wood surface machining, moisture content, and orthotropic planes of symmetry. Fiber orientation measurement was successfully performed on all the tested species. The measurement error was below 1.6°, except for oak longitudinal–radial (LR) plane showing an error up to 3.1°. For most of the species, the error was higher in LR plane because of the influence of medullary rays. Despite the observation of major variabilities in laser light scattering, it was possible to measure the grain angles with a good accuracy for all investigated species.
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