Background: the failure of the intestinal anastomosis remains one of the main problems in gastrointestinal surgery. new principles for solving this problem are focused on the cellular level of regeneration using platelet-rich plasma (prp). In this study, we evaluated the effect of PRP and experimentally compared the effectiveness of various application methods in intestinal anastomosis regeneration in rabbits. Materials and Methods: platelet-rich autoplasma was obtained from 81 rabbits, which we used to study two methods of applying prp (injection into the muscle layer/soaking in the intestinal wall) in comparison with the control group on the end-to-end small-intestinal anastomosis. We performed a morphological study of intestinal anastomoses after 24, 48, 72 hours, and after 7 days. We evaluated the deformation and strength characteristics of the anastomosis on the 7th day. Results: analysis of the histological and morphometric data of the study showed that the regeneration between the samples of intestinal anastomosis treated with prp soaking and injections into the intestinal wall is more favorable in comparison with the control group. The criteria were the quantitative ratio of inflammatory infiltrate, fibroblast proliferation, neoangiogenesis, and collagen deposition. PRP soaking in the intestinal wall significantly increased the number of adhesion conglomerates, which contributed to severe deformation and narrowing of more than ½ of the lumen in the intestinal tube. Analysis of the deformation and strength characteristics of the formed anastomoses also showed significantly higher values of the rupture strength of the anastomoses treated with prp, in contrast to the control group. Conclusion: PRP soaking and injection into the intestinal wall can have a positive effect on the regeneration of the intestinal anastomosis. PRP injection into the muscular layer of the intestinal wall significantly reduces the development of adhesions in comparison with the prp-saturated group of intestinal anastomoses. further research is needed to clarify the optimal method of prp application, which will improve the regeneration of the intestinal anastomosis.