Abstract. Detection of E6/E7 mRNA expression with real-time nucleic acid sequence-based amplification assay (NASBA) method (PreTect HPV-Proofer) from high-risk types of human papillomaviruses (HR-HPV) were compared with the presence of viral load, determined with quantitative real-time PCR in 80 cervical samples. Results regarding positivity and typing were in agreement using the two methods. However, there was no correlation between viral loads for HPV 16 or 18/45 and oncogene expression. Among 15 women with low grade atypia detected at a population-based cytology screening, and scored as 'within normal limits' according to histopathology, 14% were positive for oncogene expression, whereas 71% were HR-HPV positive. A correlation was observed between HR-HPV oncogene expression and high scores of p16 INK4a positivity. Since HPV-Proofer detects full-length E6/E7 mRNA, a positive result should correlate with presence of integrated HPV, loss of HPV replication and stabilized E6/E7 fulllength mRNA expression. Such expression from integrated HR-HPV generates a high and stable expression of full-length E6 proteins, which explains why a positive HPV-Proofer result was independent of viral load and correlate with high expression of p16 INK4a . Thus, E6/E7 oncogene expression analysis yielded information, which is consistent with and will complement the results from a real-time PCR method in a clinical prognostic procedure.

Adopting the construct of possible selves, which are conceptions of our selves in future situations, the objective of this study was to investigate how anorexia patients differ from a non-clinical control group in their conceptions of the future on qualitative content, and the four quantitative dimensions positive and negative emotional valence, and beliefs about probability and controllability. The Possible Selves Statements Test was employed. Participants presented 14 possible selves by completing the question "I can see myself …" and rating each possible self on the 4 dimensions. The patients reported a larger number of negative possible selves, with higher negative valence, often seeing future everyday situations as negative, whereas the control group saw similar situations as positive. The anorexia patients also reported negative possible selves with high controllability and high probability in relation to such situations and in some cases rated recovery from anorexia with a negative valence. Clinical implications are discussed.

The results indicate that, when compared to a non-patient group, eating disorder patients make more negative evaluations of possible selves usually seen as desirable. Depression may be a mediating factor in these evaluations for the anorexia and bulimia patients.

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