Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.

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We present here the measurement of the single-polymer entropic elasticity and the single covalent bond force profile, probed with two types of atomic force microscopes (AFM) on a synthetic polymer molecule: polymethacrylic acid in water. The conventional AFM allowed us to distinguish two types of interactions present in this system when doing force spectroscopic measurements: the first interaction is associated with adsorption sites of the polymer chains onto a bare gold surface, the second interaction is directly correlated to the rupture process of a single covalent bond. All these bridging interactions allowed us to stretch the single polymer chain and to determine the various factors playing a role in the elasticity of these molecules. To obtain a closer insight into the bond rupture process, we moved to a force sensor stable in position when measuring attractive forces. By optimizing the polymer length so as to fulfill the elastic stability conditions, we were able for the first time to map out the entire force profile associated with the cleavage of a single covalent bond. Experimental data coupled with molecular quantum mechanical calculations strongly suggest that the breaking bond is located at one end of the polymer chain.

We present experimental results on the modified Stokes force F exerted on a sphere in magnetic levitation whose position is kept fixed by an optical feedback system. A Newtonian liquid moves at a constant velocity U relative to the sphere. We consider the motion in two different situations.(i) When the sphere approaches a wall perpendicular to U, the increase in |F| due to lubrication agrees quantitatively with theoretical results such as those of Brenner (1961) and Maude (1961), obtained neglecting the unsteadiness of the flow field.(ii) In the complementary situation of a sphere moving along the axis of a cylindrical tube, our results expressed as a function of the eccentricity of the trajectory and of the ratio of the two radii confirm and extend previous theoretical analyses. They show in particular the existence of a minimum of |F| away from the axis of the cylinder and a sharp increase in |F| when the sphere approaches the sidewall. By comparing with the results for a sphere moving parallel to a flat wall, we analyse the effect of the curvature of the cylindrical tube.