Objectives: The overall goal was to analyze genetic diversity in cultivars of Musa acuminata (Colla) and M. balbisiana (Colla), commonly grown in farms from Caldas department. Scope: Characterization of the genetic variability, at the molecular and morphological level of cultivars of M. acuminata and M. balbisiana, found in farms from Caldas farmers using morphological descriptors and fluorescent microsatellites. Methodology: Phenotyping evaluations comprised 57 morphological characters following the descriptors proposed by IPGRI for the Musa genus, and for genotyping evaluations, nine fluorescent microsatellites (Simple Sequence Repeats-SSR) were used to allow the precise identification of alleles. Additionally, cluster analyses were carried out independently for both morphological and genotypic characterizations under Principal Component Analysis (PCA) and Bootstrapping methods respectively. Main results: Positive and negative highly significant correlations were found for the morphological descriptors, where traits such as presence/ absence of male bud was the rule, as well as the diameter and perimeter of this trait, plus the diameter and perimeter of the peduncle, number of fruits, pseudostem height and fruit length contributed considerably to the variability among the cultivars allowing the discrimination of three main groups in the cluster analyzes. From the molecular perspective a total of 72 polymorphic alleles were obtained, with an average genetic diversity of 0,79, polymorphic information content (PIC) of 0,77 and heterozygosity of 0,48, showed a moderate degree of genetic differentiation (FST = 0,061) among Musa cultivars, generating three main sub-clusters based on their genetic dissimilarity. Conclusions: The identification of certain morphological traits showed to be suitable for the discrimination of Musa cultivars evaluated here. On the other hand, molecular characterization allowed to establish the genetic relationships among groups, also fluorescent SSR were highly informative and accurate, in such a way that can be considered suitable for characterizations in Musa varieties.
Recibido el 12 de septiembre de 2013 y aprobado el 28 de mayo de 2014, actualizado el 10 junio de 2014
RESUMENComo consecuencia de la alimentación de Radopholus similis, se generan daños en las raíces y el cormo que conllevan a un crecimiento deficiente de las plantas y reducción en rendimiento hasta 80%. Por tal motivo, el objetivo de esta investigación fue determinar el efecto de la integración de prácticas de manejo como limpieza sanitaria de cormos y biológicas como Micorrizas Arbusculares (MA), Purpureocillium lilacinum y Bacillus subtilis sobre el crecimiento de las
Nel lavoro esegetico attorno a Lc 12 il Dottore Serafi co fa un’analisi dettagliata del contenuto e determina le due parti fondamentali di 12,1-34. La sua esegesi dei singoli passi è in concordanza con il senso del testo, e le sue osservazioni ermeneutiche, dal punto di vista teologico, filosofico e pratico, sono apprezzabili. Seguendo un metodo collaudato già dai tempi di Agostino, che poi si affermò nella scuola dei Vittorini, è frequente vedere nella sua interpretazione delle citazioni incrociate, in modo da rinforzare il ragionamento mediante testi biblici con un contenuto analogo. Quando si prende fra le mani un testo di un grande teologo come Bonaventura, non si può non ammirare la sua acutezza, il ragionamento logicamente svolto, le conclusioni e i consigli che ne ricava. Il contatto con la sua interpretazione è sempre illuminante.
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