The aim of this study was to examine the use of liposome in the dyeing of wool and mohair fibres with acid dyestuffs. Soybean lecithin and cholesterol were used to form the liposome membrane utilised in the dyebath. Liposome production was performed according to the thin lipid layer method (Bangham Method) using a rotary evaporator. Two different forms of liposome were used for dyeing wool and mohair fibres. In its first form, liposome was utilised as an auxiliary agent, where it was added to a conventional dyebath at the beginning of the process. In its second form, dyes were encapsulated with liposome and then used in dyeing. The effects of these two different forms of liposome were compared with conventional dyeing. Dyeing was carried out at depths of shade of 0.5%, 1.0% and 2.0% using three different concentrations of liposome (0.33%, 0.66% and 1.33%). An analysis of K/S values, fastness to washing, and the alkali solubility of fibres was conducted. The fibre samples dyed in the presence of liposome exhibited very good fastness to light (grade 8). The wash fastness test results of the liposomal‐dyed samples were significantly better (grade 4‐5) than for those samples which were conventionally dyed. In the presence of liposome, the tensile strength of fibres was 20 gf, whereas it was 11 gf without liposomes.

Glucose oxidase is a type of enzyme that converts glucose into hydrogen peroxide and gluconic acid by enzymatic reaction. Glucose oxidase is widely used in industry; however, in the textile industry, glucose oxidase has only received academic interest. Previously, wool was bleached by some reducing agents; however, currently in industry, hydrogen peroxide dominates the bleaching of wool fibres. In this study, the effect of glucose oxidase enzyme treatment on wool merino fibres and dyeability properties was investigated. Wool fibres were treated with glucose oxidase enzyme, after which the whiteness index (Stensby) and yellowness index (ASTM D 1925 and ASTM E 313) were investigated. Scanning electron microscopy and scanning electron microscopy with energy dispersive X-ray spectroscopy were used to identify the morphological structure of wool fibres and their atomic content. The chemical damage caused by enzyme was investigated using a fluorescence and a light microscope, and the alkali solubility (ASTM D 1283) was determined. After enzymatic treatment, the wool fibres were dyed at a 2.0% concentration with reactive dyes. Dyeability (K/S) and CIELab values were assessed with a Minolta CM 3600 D spectrophotometer (D65, 10 ). The washing fastness of wool fibres was investigated according to TS EN ISO 105-C06 (A1S).

This research investigated the effect of various proteolytic enzymatic pretreatment on morphological and chemical features and the dyeability properties of wool fibres. Scoured merino wool fibres are treated with protease, papain, trypsin, and pepsin in specified conditions. Each enzyme activity measurement was provided by appropriate methods such as Bradford, BAPNA (N-benzoyl-1-arginine-p-nitroanilide), and BSA (Bovine Serum Albumin). Enzymatic processes were carried out for 24 h in the incubator set at 40 C, 100 rpm, and specified pH with 1 mg/ml enzyme concentration. Whiteness index (Stensby) and yellowness index (ASTM D 1925) were examined after enzymatic pretreatment. Pepsin and trypsin-treated wool fibres showed the highest whiteness index as 61.3 and 61.1, respectively whilst untreated wool fibres had 52.2. Fourier-transform infrared (FTIR) analysis revealed the increase in the intensity of amide-related bands and hydroxyl bands after enzymatic treatment.Scanning electron microscopy (SEM) photomicrographs manifested the cuticle layer is partially removed in enzyme-treated fibres. Elemental identification was provided by SEM-energy-dispersive X-ray spectroscopy (EDX). It appears that the sulphur bonds decreased after the treatment and the pepsin-treated fibres have fewer bonds of all. To examine the damage to the structure, photomicrographs were taken using fluorescence and light microscopes. The alkali solubility test (ASTM D1283) was also conducted to compare different enzyme types. Wool fibres were dyed in 2.0% concentration with reactive dyestuff. Dyeability and colorimetric features of fibres were measured by a spectrophotometer. The washing fastness test showed that all the samples have good results and the colour change after washing was better in enzyme-treated samples (grade 5) compared to untreated wool fibres (grade 4-5).