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SUMMARY:Endothelial cells in culture can be seeded onto a variety of surfaces so as to yield a confluent monolayer. In some instances the surface required for experimental or clinical applications may be hard to endothelialize. Special techniques for seeding endothelial cells onto filters, grafts, and tubes are described. Adaptations of these techniques may allow endothelial cells to be introduced onto a variety of artificial biomaterials and prostheses.