Population-level morphometric variation of the Mountain Mullet (Agonostomus monticola) was assessed in 419 adult specimens from 25 sample sites (river basins) across its Middle American distribution (Pacific and Atlantic-Caribbean drainages). This analysis was based on 36 standardized linear measurements and 19 landmarks on geometric morphometrics approach. Discriminant function analysis (DFA) revealed 19 linear morphological characters with significant variation among groups. Geometrically, the most notable changes were associated to the curvature of the frontal region of the head, the anterior and posterior insertion of the first dorsal and anal fins. The resulting grouping based on the DFA and geometric morphometrics techniques (Pacific-A, Pacific-B and NE México-Caribbean) were similar to those previously recovered by genetic techniques, where the Pacific-B (Ayuquila river basin) was the most different group. Our results provide morphological evidence for considering Agonostomus monticola as a complex of evolutionary entities, represented by two forms in the Pacific Ocean and another in the Atlantic Ocean.Keywords: Discriminant analysis, Evolutionary entities, Landmarks, Middle American distribution, Morphometry. La variación morfométrica de poblaciones de la lisa de montaña Agonostomus monticola fue evaluada en 419 especímenes adultos recolectados en 25 sitios (cuencas) a través de su distribución mesoamericana (Pacífico, Atlántico y cuencas del Caribe). El análisis fue basado en 36 medidas lineales estandarizadas y 19 puntos de referencia basados en morfometría geométrica. El análisis de función discriminante (AFD) reveló 19 caracteres morfométricos con variación significativa entre grupos. Geométricamente, los cambios más notables estuvieron asociados con la curvatura de la región frontal de la cabeza y la inserción anterior y posterior de la primera aleta dorsal y de la aleta anal. Los agrupamientos resultantes del AFD y del análisis de morfometría geométrica (Pacífico-A, Pacífico-B y NE México-Caribe) fueron similares con los previamente definidos mediante técnicas genéticas. El grupo Pacífico-B (cuenca de Ayuquila) fue el más disímil. Nuestros resultados proveen evidencia morfológica para considerar a Agonostomus monticola como un complejo de entidades evolutivas, representadas por dos formas en la cuenca del océano Pacífico y una forma en la cuenca del océano Atlántico.
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