Root chicory (Cichorium intybus var. sativum) is a cash crop cultivated for inulin production in Western Europe. This plant can be exposed to severe water stress during the last 3 months of its 6-month growing period. The aim of this study was to quantify the effect of a progressive decline in water availability on plant growth, photosynthesis, and sugar metabolism and to determine its impact on inulin production. Water stress drastically decreased fresh and dry root weight, leaf number, total leaf area, and stomatal conductance. Stressed plants, however, increased their water-use efficiency and leaf soluble sugar concentration, decreased the shoot-to-root ratio and lowered their osmotic potential. Despite a decrease in photosynthetic pigments, the photosynthesis light phase remained unaffected under water stress. Water stress increased sucrose phosphate synthase activity in the leaves but not in the roots. Water stress inhibited sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1 fructosyltransferase after 19 weeks of culture and slightly increased fructan 1-exohydrolase activity. The root inulin concentration, expressed on a dry-weight basis, and the mean degree of polymerization of the inulin chain remained unaffected by water stress. Root chicory displayed resistance to water stress, but that resistance was obtained at the expense of growth, which in turn led to a significant decrease in inulin production.

Abstract.A good understanding of the soil water content (SWC) distribution at the field scale is essential to improve the management of water, soil and crops. Recent studies proved that Electrical Resistivity Tomography (ERT) opens interesting perspectives in the determination of the SWC distribution in 3 dimensions (3-D). This study was conducted (i) to check and validate how ERT is able to monitor SWC distribution in a maize field during the late growing season; and (ii) to investigate how maize plants and rainfall affect the dynamics of SWC distribution. Time Domain Reflectometry (TDR) measurements were used to validate ERT-inverted SWC values. Evolution of water mass balance was also calculated to check whether ERT was capable of giving a reliable estimate of soil water stock evolution. It is observed that ERT was able to give the same average SWC as TDR (R 2 = 0.98). In addition, ERT gives better estimates of the water stock than TDR thanks to its higher spatial resolution. The high resolution of ERT measurements also allows for the discrimination of SWC heterogeneities. The SWC distribution showed that alternation of maize rows and inter-rows was the main influencing factor of the SWC distribution. The drying patterns were linked to the root profiles, with drier zones under the maize rows. During short periods, with negligible rainfall, the SWC decrease took place mainly in the two upper soil horizons and in the inter-row area. In contrast, rainfall increased the SWC mostly under the maize rows and in the upper soil layer. Nevertheless, the total amount of rainfall during the growing season was not sufficient to modify the SWC patterns induced by the maize rows. During the experimental time, there was hardly any SWC redistribution from maize rows to inter-rows. Yet, lateral redistribution from inter-rows to maize rows induced by potential gradient generates SWC decrease in the inter-row area and in the deeper soil horizons.

Environmental stresses such as salinity directly impact crop growth, and by extension, world food supply and societal prosperity. It is estimated that over 800 million hectares of land throughout the world are salt-affected. In arid and semi-arid regions, salt concentration can be close to that in the seawater. Hence, there are intensive efforts to improve plant tolerance to salinity and other environmental stressors. Salicylic acid (SA) is an important signal molecule for modulating plant responses to stress. In the present study, we examined, on multiple plant growth related endpoints, whether SA applied through the rooting medium could mitigate the adverse effects of salinity on tomato (Solanum lycopersicum) cv. Marmande. The latter is a hitherto understudied tomato plant from the above perspective; it is a classic variety that produces the large ribbed tomatoes in the Mediterranean and consumed worldwide. We found salt stress negatively affected the growth of cv. Marmande tomato plants. However, the SA-treated plants had greater shoot and root dry mass, leaf area compared to untreated plants when exposed to salt stress. Application of SA restores photosynthetic rates and photosynthetic pigment levels under salt (NaCl) exposure. Leaf water, osmotic potential, stomatal conductance transpiration rate, and biochemical parameters were also ameliorated in SA-treated plants under saline stress conditions. Overall, these data illustrate that SA increases cv. Marmande tomato growth by improving photosynthesis, regulation and balance of osmotic potential, induction of compatible osmolyte metabolism, and alleviating membrane damage. We suggest salicylic acid might be considered as a potential growth regulator to improve tomato plant salinity stress resistance, in the current era of global climate change.

Root Water Uptake Dynamics of Cichorium intybus var. sa vum Under Water-Limited Condii onsChicory (Cichorium intybus L.) is a cash crop culi vated in Western Europe for inulin produci on. Due to actual and future climate changes, this plant could be exposed to severe water stress at the end of its growing period, leading to a decrease of its yield. The aim of this work was to invesi gate the chicory root water uptake dynamics and the plant ability to compensate a lack of water in the upper horizons. We performed a controlled experiment with 3 replicates under contrasted irrigai on scenarios. We observed that, in case of drought, total root length decreased and root profi les developed deeper. We successfully used a one-dimensional Richards-based model with a stress funci on and a compensai on mechanism (Hydrus 1-D) to inversely characterize the dynamics of the actual sink-term profi les under both irrigai on scenarios. We could also use the model to assess the compensai on thanks to a weighted stress index that is consistent between replicates. The extraci on profi les evolved diff erently under water-defi cit and controlled situai ons. The passive compensai on mechanism allowed chicory roots under water-limited condii ons to take water deeper in the soil, where they had only few lateral roots. We found that, in case of drought, compensai on started before the plants had to reduce their transpirai on rate. Because the soil kept drying out, compensai on was not suffi cient anymore, and the plants had to decrease their transpirai on some days later. However, chicories maintained their metabolism and coni nued to transpire and to growth slowly. This allowed them to adapt thanks to an aci ve compensai on mechanism, by generai ng new lateral roots in wet er horizons. This study also showed that there was no unique Feddes stress parameter set able to describe plant behavior under contrasted irrigai on condii ons or even under diff erent plant development stages.Abbreviai ons: DAS, days a er sowing; IRGA, InfraRed Gaz Analyzer; RMSE, root mean square error; TDR, i me domain refl ectrometry.

Abstract. A good understanding of the soil water content (SWC) distribution at the field scale is essential to improve the management of water, soil and crops. Recent studies proved that electrical resistivity tomography (ERT) opens interesting perspectives in the determination of the SWC distribution in 3 dimensions (3-D). We conducted this study (1) to check and validate the sensitivity of ERT for monitoring SWC distribution in a maize field during the late growing season; and (2) to investigate how maize plants and precipitations affect the dynamics of SWC distribution. We used time domain reflectometry (TDR) measurements to validate ERT-inverted SWC values. We also calculated the evolution of water mass balance to check whether ERT was capable of giving a reliable estimate of soil water stock evolution. We observe that ERT is able to give the same average SWC as TDR (R2 = 0.98). In addition, we showed that ERT give better estimates of the water stock than TDR thanks to its higher spatial resolution. The high resolution of ERT measurements also allows the discrimination of SWC heterogeneities. The SWC distribution shows that alternation of maize rows and inter-rows is the main influencing factor of the SWC distribution. The drying patterns are linked to the root profiles, with drier zones under the maize rows. During small dry periods, the SWC decrease occurs mainly in the two upper soil horizons and in the inter-row area. At the opposite, precipitations increase the SWC mostly under the maize rows and at the upper soil layer. Nevertheless, the total amount of rainfall during the growing season is not sufficient to modify the SWC patterns induced by the maize rows. During the experimental time, the SWC redistribution hardly occurred from maize rows to the inter-rows but lateral redistribution from the inter-row to the maize rows induced by potential gradient generates SWC decrease in the inter-rows area and in the deeper soil horizons.