Penetration of near infrared radiation (NIR) through biological tissues (human wrist, muscle and skin of hen, muscle of mollusk) was studied. The possibilities of visualizing various objects within biological tissues were examined. It is found that: (a) it is possible to see small objects with millimeter and sub-millimeter dimensions; and (b) by using NIR rays it is possible to distinguish different parts of small homogeneous biological tissues. NIR can be a possible tool in the near future to visualize millimeter size cancerous outgrowths located inside human body cavities. It will enable diagnosis of cancer at an early stage of development.
During exposure to the cell phone electromagnetic field (EMF), some neurons in the brain at areas of peak specific absorption rate (SAR) absorb more electromagnetic energy than is permitted by existing guidelines. The goal of the present work was to investigate the influence of cell phone-like EMF signal on excitability and memory processes in single neurons. A Transverse Electromagnetic Cell (TEM Cell) was used to expose single neurons of mollusk to the EMF. Finite-Difference Time-Domain (FDTD) method was used for modeling the TEM Cell and the EMF interactions with living nerve ganglion and neurons. Neuron electrophysiology was investigated using standard microelectrode technique. SAR deposited into the single neuron was calculated to be 8.2 W/kg with a temperature increment of 1.21°C. After acute exposure, the threshold of firing of action potentials (AP) was significantly decreased (p ≈ 0.001). Time of habituation to stimulation with the intracellular current injection was increased (p ≈ 0.003). These results indicate that acute exposure to EMF at high SARs impairs the ability of neurons to store information.
The aim of present work is to explore the influence of extremely low-frequency electromagnetic fields (8.34 and 217 Hz) utilized in cell phones on habituation of the mollusk single neuron to intracellular stimuli. The isolated nervous system of the mollusk Helix Pomatia was used in the experiments. Helmholtz coils were used to expose brain ganglia to the low-frequency electromagnetic fields. Peak values of the extremely low-frequency fields were between 1 and 6 mT. Neuron electrophysiology was investigated using a standard microelectrode technique. Exposure of the neuron to the low-frequency electromagnetic fields caused dehabituation to intracellular stimulus. The effect was proportional to the magnetic induction peak value. The observed dehabituation occurs by degradation of the signal to noise ratio and by alteration of the neuron's normal function.
Background: Imaging plays a crucial role in the identification, localization and grading of prostate carcinoma. However current imaging methods for the prostate cancer diagnosis are complicated and partially invasive. The drawback of existent imaging method is that they cannot detect prostate carcinoma at early stage of development. Therefore, a key challenge for prostate cancer detection is to use a simple and noninvasive method which will able to detect the cancer with very small dimensions. Correspondingly, the aim of present work was to demonstrate the possibility of using a near infrared light for the prostate cancer detection in vitro. Methods: Experiments were carried out on prostates obtained after radical prostatectomy. Infrared light emitted diodes were used as illumination sources. Infrared light passing through the prostate was caught by charge-coupled device connected to computer and prostate infrared transillumination images were obtained. Results: Intensity of near infrared light passing through the noncancerous prostate tissue is nearly homogeneous. Intensity of near infrared light passing through the cancerous outgrowth is lower than the intensity passing through the non-cancerous tissue of the same prostate, thereby cancerous formations are differentiated as the dark areas on the relatively white background. Specially developed software analyzes and processes distribution of intensities of the grayscale images, measures the ratio of their strength, and determines the rate of prostate malignancy. Conclusions: Obtained results may hold some promise to make an important contribution to the diagnosis of prostate cancer in early stage of its development.
We have experimentally demonstrated that the emission of visible light from the polymer matrix doped with luminescent dye and gold nanoparticles (GNPs) can be enhanced with the use of surface plasmon coupling. GNPs can enhance the luminescence intensity of nearby luminescent dye because of the interactions between the dipole moments of the dye and the surface plasmon field of the GNPs. The electric charge on the GNPs and the distance between GNPs and luminescent dye molecules have a significant effect on the luminescence intensity, and this enhancement depends strongly upon the excitation wavelength of the pumping laser source. In particular, by matching the plasmon frequency of GNPs to the frequency of the laser light source we have observed a strong luminescence enhancement of the nanocomposite consisting of GNPs coupled with luminescent dye Nile blue 690 perchlorate. This ability of controlling luminescence can be beneficially used in developing contrast agents for highly sensitive and specific optical sensing and imaging. This opens new possibilities for plasmonic applications in the solar energy field.
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