No abstract

Abstract:The effects of the decoction of Erythrina velutina Willd., Fabaceae, were investigated using the root meristem cells of Allium cepa L., Amaryllidaceae. Ten concentrations of the aqueous extract (0.125 to 1.25%) of this medicinal plant were analyzed at both macroscopic and microscopic levels. All concentrations showed root growth inhibition after 96 h treatment. Although there were no signifi cant differences between the mitotic indexes of any concentration and the control, there were changes in the frequencies of cell stages at three different concentrations. Additionally, the presence of fi ve different cells abnormalities was recorded: chromosome bridging, lagging chromosomes, chromosome fragments, disturbed metaphase and disturbed anaphase. These results suggest inhibitory and genotoxic activity of the decoction of E. velutina on Allium cepa.

Cytogenetic study of Leptodactylus fuscus and L. latrans (Anura: Leptodactylidae) from the semiarid Brazilian Caatinga scrublands. Frogs in the family Leptodactylidae have diploid chromosome numbers ranging from 2n = 18 to 2n = 26, although there is a predominance of 2n = 22 karyotypes. In the present study, 52 specimens of Leptodactylus fuscus and Leptodactylus latrans were obtained from three municipalities (Carira, Poço Redondo, and Tobias Barreto) of the Brazilian state of Sergipe for karyotype analysis using conventional Giemsa staining techniques, and C-and Ag-NOR banding of the chromosomes obtained from the intestinal epithelium. The results of this study show that the individuals analyzed have highly similar karyotypes, with no evidence of any pronounced species-specific markers. However, some differences were observed in the chromosome morphology and C-bands in comparison with the karyotypes described previously for these species, which may represent intraspecific geographic variation in both taxa.Keywords: amphibians, chromosomes, C-bands, Ag-NOR. 126Phyllomedusa -12(2), December 2013 Resumo Análise citogenética de Leptodactylus fuscus e L. latrans (Anura: Leptodactylidae) de áreas de Caatinga do Brasil. A família Leptodactylidae apresenta números diplóides que variam de 2n = 18 a 2n = 26, porém, na maioria dos gêneros descritos na literatura, há uma predominância de cariótipos com 2n = 22. Neste trabalho foram analisados citogeneticamente 52 exemplares de Leptodactylus fuscus e Leptodactylus latrans coletados pela primeira vez no estado de Sergipe, norte do Brasil, nos municípios de Carira, Poço Redondo e Tobias Barreto, por meio de técnicas de coloração convencional, Banda C e Ag-RON, empregadas em preparações do epitélio intestinal. Os dados mostraram que as espécies aqui analisadas apresentaram padrões cariotípicos muito similares, sem evidência de marcadores específicos das espécies. Contudo, algumas variações de morfologia cromossômica e bandas C observadas divergiram de cariótipos já descritos na literatura e podem indicar diferenças cromossômicas geográficas intraespecíficas para ambas as espécies.

No abstract

No abstract