This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 35A c c e p t e d M a n u s c r i p t Trichinella introduction within mainland Australia, which is considered Trichinella-free. Sera were 35 examined using both an 'in-house' and a commercially available indirect-ELISA that used other parasitic infections, more work using well-defined cohorts of positive and negative samples is 53 required. Even if the specificity of the ELISAs is proven to be low, their ability to correctly classify 54 the small number of true positive sera in this study indicates utility in screening wild boar 55 populations for reactive sera which can be followed up with additional testing.56 57

No abstract

Light trap surveillance across northern Australia and Papua New Guinea (PNG) has detected the presence of several Oriental species of Culicoides not previously reported from those countries and which appear to have arrived in recent times. Detections of C. nudipalpis Delfinado in Western Australia, C. flavipunctatus Kitaoka and C. palpifer Das Gupta and Ghosh in the Northern Territory and of C. flavipunctatus, C. fulvus Sen and Das Gupta and C. orientalis Macfie in Queensland (Qld) provide evidence of multiple pathways for incursions of biting midges into northern Australia. Of these, only C. fulvus appears to have established. Additionally, three species, C. fulvus, C. wadai Kitaoka and C. brevipalpis Delfinado, are newly reported from PNG and all appear to be well established. The arrival in PNG of C. fulvus and C. brevipalpis, both not previously reported from Qld, suggests that pathways exist for the entry of Oriental insects into New Guinea directly from Asia, rather than via Australia. Molecular analyses using DNA barcodes (partial mitochondrial cytochrome oxidase subunit one sequences) confirmed morphological identification of specimens and additionally provided strong evidence relating to the source of these incursions. At least two of these species are vectors of important livestock viruses and are likely to impact on the epidemiology of these viruses as they continue to disperse.