The aim of this study was to assess the relationship between the level of physical activity, climacteric symptoms and health-related quality of life in postmenopausal women. The cross-sectional study included 100 postmenopausal women using a public leisure park. A questionnaire regarding the sociodemographic and clinical characteristics was applied. The Menopause Rating Scale (MRS) was used to assess climacteric symptoms and health-related quality of life, and the International Physical Activity Questionnaire (IPAQ) (short version) to estimate the level of physical activity. The average age of women was 56.8 years. Insufficiently active women had higher overall score and MRS domains than physically active ones, indicating higher intensity of climacteric symptoms (<0.001). Most (83.3%) of physically active women had a higher level of health-related quality of life, while in the insufficiently active group, only 10% had this condition (<0.001). Active women were 10.6 times more likely to have better health-related quality of life than insufficiently active women. Regular physical activity seems to contribute positively to the reduction of climacteric symptoms and better health-related quality of life in postmenopausal women.Keywords: menopause, physical activity, quality of life.