The effective control of an insect pest such as Drosophila suzukii (Diptera: Drosophilidae) has been challenging because its attack on red fruits can cause losses in production of up to 80% worldwide. The objective of this work was to extract and characterise the essential oil from rosemary (Rosmarinus officinalis), produce and characterise nanoparticles incorporated with this essential oil, evaluate the in vivo insecticide activity of free and nanoencapsulated essential oil against D. suzukii, and evaluate the effect of that oil on the enzyme acetylcholinesterase. The nanoparticles produced with the essential oil from rosemary had a particle size and surface charge of 236.6 nm and À11.8 mV, respectively. According to the FTIR data, the essential oil was incorporated into the polymeric matrix of poly(ε-caprolactone). Through in vivo toxicity analysis, the free and nanoencapsulated essential oils were found to possess insecticidal activity, with an LD 50 close to 9.1 g/L. The maximum anticholinesterase activity for the essential oil was 75% at a concentration of 0.10 mg/mL. The incorporation of the essential oil from R. officinalis into biodegradable nanoparticles of poly(ε-caprolactone) potentiated and prolonged its in vivo insecticidal activity against D. suzukii.
RESUMOObjetiva-se com este trabalho relatar e analisar uma aula desenvolvida na disciplina de metodologia de ensino de Ciências ofertada pelo curso de licenciatura em Ciências Biológicas da Universidade Federal de Lavras, MG. A aula buscou construir conceitos referentes aos movimentos internos da Terra utilizando como estratégia metodológica uma peça teatral, baseada na mitologia da cultura chilena do povo Mapuche. Através desta ferramenta pedagógica foi possível compreender suas relações com o processo de ensino aprendizagem de ciências e trabalhar com a diversidade cultural. A aula foi aplicada aos licenciandos que cursavam a disciplina e, a partir da análise feita por eles, percebe-se que o uso da dramatização permitiu abordar o tema de uma forma mais dinâmica e assim construir os conceitos propostos de maneira mais interativa e coletiva.
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