The clinical applications of magnetic hyperthermia therapy (MHT) have been largely hindered by the poor magnetic-to-thermal conversion efficiency of MHT agents. Herein, we develop a facile and efficient strategy for engineering encapsulin-produced magnetic iron oxide nanocomposites (eMIONs) via a green biomineralization procedure. We demonstrate that eMIONs have excellent magnetic saturation and remnant magnetization properties, featuring superior magnetic-to-thermal conversion efficiency with an ultrahigh specific absorption rate of 2390 W/g to overcome the critical issues of MHT. We also show that eMIONs act as a nanozyme and have enhanced catalase-like activity in the presence of an alternative magnetic field, leading to tumor angiogenesis inhibition with a corresponding sharp decrease in the expression of HIF-1α. The inherent excellent magnetic-heat capability, coupled with catalysis-triggered tumor suppression, allows eMIONs to provide an MRI-guided magneto-catalytic combination therapy, which may open up a new avenue for bench-to-bed translational research of MHT.

Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly( l -lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO 2 ). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering.

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In recent times, the copper chalcogenide (Cu2–x E, E = S, Se, Te, 0 ≤ x ≤ 1)-based nanomaterials have emerged as potent photothermal agents for photothermal therapy (PTT) because of their advantageous features, such as the low cost, reduced toxicity, biodegradability, and strong absorption of near-infrared (NIR) light in a relatively wide range of wavelength. Nevertheless, the applicability of Cu2–x E-based PTT is limited because of its inadequate photothermal conversion efficiency, as well as insufficient destruction of the tumor area unexposed to the NIR laser. Fortunately, Cu2–x E nanomaterials also act as photosensitizers or Fenton-reaction catalysts to produce reactive oxygen species (ROS), referring to ROS-related therapy (RRT), which could further eradicate cancer cells to address the aforementioned limitations of PTT. Moreover, PTT improves RRT based on photodynamic therapy (PDT), sonodynamic therapy (SDT), chemodynamic therapy (CDT), and radiotherapy (RT) in different ways. Inspired by these facts, integrating Cu2–x E-based PTT with RRT into a single nanoplatform seems an ideal strategy to achieve synergistically therapeutic effects for cancer treatment. Herein, we discuss the synergetic mechanisms, composition, and performances of recent nanoplatforms for the combination of Cu2–x E-based PTT and RRT. In addition, we give a brief overview on some specific strategies for the further improvement of Cu2–x E-based PTT and RRT combined cancer treatment to enable the complete eradication of cancer cells, such as realizing the imaging-guided synergistic therapy, promoting deep tumor penetration of the nanosystems, and boosting O2 or H2O2 in the tumor microenvironment. Finally, we summarize with intriguing perspectives, focusing on the future tendencies for their clinical application.

Despite the success and advantages over traditional chemotherapeutic strategies, photothermal therapy (PTT) suffers from certain limitations, such as poor stability, low therapeutic efficacy of PTT agents in vivo, and their affinity loss during the multistep synthesis process of delivery carriers, among others. To address these limitations, we designed a stable, biocompatible, and dual-triggered formulation of indocyanine green (ICG)-encapsulated silk fibroin (SF) (ICG-SF) nanoparticles using supercritical fluid (SCF) technology. We demonstrated that ICG encapsulation in SF through this environmental-friendly approach has offered excellent photothermal stability, the pH-responsive release of ICG from SF specifically in the tumor acidic environment, and its substantial activation with near-infrared (NIR) light at 808 nm significantly enhanced the PTT efficiency. In vitro and in vivo photothermal experiments have shown that these ICG-SF nanoparticles were capable of devastating tumor cells merely under light-induced hyperthermia. Together, these results have suggested that the biocompatible ICG-SF nanoparticles prepared by the SCF process resulted in high PTT efficiency and may have a great potential as a delivery system for sustained cancer therapy.