Green synthesis of metal nanoparticles (NP) has emerged as a recent trend in green chemistry. However, their potential in the field of agriculture is yet underexplored. In this study, silver nanoparticles (Ag NPs) were synthesized by using the fruit juice and peel extract of Citrus maxima (Burm.) Merr. as a reducing and capping agent. The nano-primed seeds of Zea mays, Glycine max, and Cicer arietinum showed enhanced germination rate up to 90% as compared to the respective control (30-60%) and also enhanced plant growth. The HRTEM images of fruit-mediated Ag NPs showed well-dispersed particles. The XRD diffraction pattern confirmed the crystalline nature of Ag NPs with fcc lattice points of 101, 111, 200, 220, and 311 with an average crystal size of 19.1275±1.73 nm. On the other hand, the peel-mediated Ag NPs exhibited a size range of 10-20 nm with average crystal size of 19.09±1.71 nm. The FTIR spectra confirmed the presence of ʋC=C-H, ʋCH2, ʋC=O (stretch), ʋC=C (stretch), ʋC-H (bend), ʋC-O (bend) in the Ag NPs synthesized by the fruit and peel extract. The Ag NPs also showed potential antimicrobial effects against both Gram-positive (Staphylococcus aureus) and negative bacteria (Klebsiella pneumonia).

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The present study aims at exploring the microbial diversity in one of the most important medicinal plant- Cissus quadrangularis Linn. Stems and leaves of the plant under study were collected from three districts of Assam. The study was conducted in two different seasons-summer and winter. 16 endophytic fungal species were isolated from the studied samples and were morphologically identified of which 5 were sterile ones. The isolation of endophytic bacteria was done on Nutrient Agar media using standard protocols. Based on the amplification of 16S rDNA amplification and comparing the results with the BLAST results and the phylogenetic tree, the organisms were identified to be- Bacillus subtilis and Bacillus velenzensis. This study will give further scope for studying the secondary metabolites from the Endophytic isolates of this plant and establish relationship for its bone healing ability.