Background:Calcium sandoz-250 is an Ayurvedic calcium supplement, containing Khatika Churna. Bioavailability study of the formulation is essential for estimation of peak plasma concentration (Cmax), time to Cmax and rate of absorption.Aim:To evaluate the absorption parameters of calcium sandoz-250 in albino rats by atomic absorption spectroscopic (AAS) method.Materials and Methods:Study was carried out as a single dose, open-label, randomized study. Estimation of calcium was carried out by AAS, after validating the method for a few parameters for the estimation. Pharmacokinetic parameters such as Cmax, time to peak concentration (Tmax), area under the plasma concentration - time curve were calculated for calcium on administration of calcium sandoz-250.Results:Linearity curve was plotted for 0.5-2.5 ppm, given R2 value 0.9975. The Cmax, i.e. Cmax after administration of calcium sandoz-250 was 0.793 mg/ml at 90 min (Tmax). Measurable calcium-blood levels were noticed in all subjects up to 3.0 h after administration of calcium sandoz-250.Conclusion:Calcium sandoz-250, consisting of Khatika Churna, increases the blood calcium level in albino rats.