Effect of ethanol extract of the leaf of plants Hydrocotyl asiatica, Boerhavia diffusa, Bacopa monieri and Trichosanthes cucumarina was tested against the pulse beetle Callosobruchus chinensis. The efficacy of the extract on the insects was dose-dependent. Different doses were checked and the results showed that high doses of the extracts were significantly more toxic to Callosobruchus chinensis compared to lower doses. LD50 value was assessed using probit analysis. The effect of the plant Hydrocotyl asiatica was found to be most significant causing highest mortality compared to other plants. DNA fragmentation studies also showed similar results and points out the significance of the medicinal plants in controlling the pest.

Aqueous, ethanol and acetone leaf extracts of the four medicinally important plants Vetiveria zizanoides, Asparagus racemosus, Hyptis sauveolens and Citrus limon was used to demonstrate their toxic effects on the blood sugar trehalose of the stored insect pest Callosobruchus chinensis. The plant leaves caused significant changes on the amount of trehalose and the effect was dose-dependent. Trehalose was found to be decreased compared to control insects. Acetone extract of Citrus limon at sub-lethal dose (1.6%) could produce significant effects compared to aqueous and ethanol extracts indicating the presence of more active components in it. The medicinal plants thus proved to have strong insecticidal activity against the insect pests. Citation: Ganga S, Bindhu VR, Dayanandan S (2017) Effect of Medicinal Plant Extracts on the Blood Sugar Trehalose of the Pulse BeetleCallosobruchus Chinensis (Coleoptera: Bruchide). J Fertil Pestic 7: 175.