3,3',4',5-Tetrachlorosalicylanilide (TCS) is an effective metabolic uncoupler utilized for microbial yield reduction. However, its potential impact, in particular on the soluble microbial products (SMP) formation, is unknown yet. Herein we study the effect of TCS on SMP production and analyze the related mechanism. The addition of TCS in activated sludge system led to an increased production of SMP, especially proteins. The SMP were produced in proportion to the substrate utilization at a low TCS concentration, while more non-substrate-associated SMP were released at a high TCS concentration. TCS simulated the production of extracellular polymeric substances (EPS) and enhanced cell lysis, which both contributed to SMP production. FTIR and EEM analyses show that the SMP, EPS, and cell lysis products have similar functional groups and fluorescence properties, indicating a similar origin of these substances. In addition, a dose of TCS increased the release of high molecular weight compounds due to cell lysis. This study might benefit for a better understanding of the response of activated sludge to metabolic uncouplers like TCS.

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We analyze 10 years of Fermi-LAT data towards the SS433/W50 region. With the latest source catalog and diffuse background models, the γ-ray excess from SS433/W50 is detected with a significance of ∼ 6σ in the photon energy range of 500 MeV -10 GeV. Our analysis indicates that an extended flat disk morphology is preferred over a point-source description, suggesting that the GeV emission region is much larger than that of the TeV emission detected by HAWC. The size of the GeV emission is instead consistent with the extent of the radio nebula W50, a supernova remnant being distorted by the jets, so we suggest that the GeV emission may originate from this supernova remnant. The spectral result of the GeV emission is also consistent with an supernova remnant origin. We also derive the GeV flux upper limits on the TeV emission region, which put moderate constrains on the leptonic models to explain the multiwavelength data.