LncRNA-NEF is a tumor suppressor lncRNA in liver cancer. The present study aimed to investigate the role of lncRNA-NEF in intrahepatic cholangiocarcinoma (IHCC), which is second most common type of primary cancer of the hepatobiliary system that causes high mortality rate. In the present study we found that lncRNA-NEF was down-regulated, while Runt-related transcription factor 1 (RUNX1) was up-regulated in tumor tissues than in adjacent healthy tissues of IHCC patients. Expression levels of lncRNA-NEF and RUNX1 were significantly and reversely correlated in tumor tissues but not in adjacent healthy tissues. Plasma levels of lncRNA-NEF were significantly lower in IHCC patients than in healthy controls. Down-regulation of lncRNA-NEF effectively distinguished stage I and II IHCC patients from healthy controls. Patients were followed up for 5 years, patients with high plasma levels of lncRNA-NEF showed significantly better survival conditions compared with patients with low expression levels of lncRNA-NEF. LncRNA-NEF overexpression led to inhibited expression of RUNX1 in cells of IHCC cell lines and inhibited cancer cell migration and invasion. In contrast, RUNX1 overexpression showed no significant effects on lncRNA-NEF expression, but attenuated the effects of lncRNA-NEF overexpression on cancer cell migration and invasion. We therefore concluded that lncRNA-NEF participated in IHCC possibly by interacting with RUNX1.

Using photocatalysis to produce hydrogen is a promising research area in the immediate future. This article intends to design photocatalysts of crystalline (amorphous) TiO 2 /two-dimensional MoS 2 sandwich structure supporting singleatoms (SAs). After the effective mass, electrostatic potential, light absorption spectrum and other related properties were calculated, the analysis shows that catalysts with amorphous sandwich structure has excellent electron-hole separation ability, and precious metal SAs can adsord onto the amorphous sandwich structure stably. Except for the Pt@Amorphousr-TiO 2 /MoS 2 sandwich structure single-atom catalysts (SACs), which is only active towards oxygen evolution, all the other interlayer catalysts are active for both photocatalytic hydrogen evolution and oxygen evolution.

The preparation of hydrogen by photocatalytic principle is an important subject in the future energy direction. This article intends to design the catalytic structure of a crystalline (amorphous) TiO2/two-dimensional MoS2 sandwich structure supporting a single-atom, and explore the effect of this special structure on the photocatalytic reaction.Calculating the effective mass, electrostatic potential, light absorption spectrum and other related properties by DFT method, the analysis shows that the sandwich structure catalyst has good carrier transfer ability and electron-hole separation ability. Except for the Pt@Amorphousr-TiO2/MoS2 sandwich structure single-atom catalyst, which only has an excellent effect on the oxygen evolution of water, the rest of the interlayer catalysts have the two characteristics of photocatalytic hydrogen evolution and oxygen evolution.The new catalyst designed in this paper has innovative design ideas and high-efficiency research and development value.