Objective: The aim of the study was to compare emotion regulation among the authoritative, authoritarian and permissive parenting styles of mothers with preschool children. Methods:The statistic community of this study was all the mothers in Tehran who had preschool children aged between 4 and 6 years. By convenient sampling, 8 kindergartens were selected, and the questionnaires were completed by mothers. The sample consisted of 270 mothers with preschool children. The participants completed the questionnaires, and the data were analyzed with MANOVA. Results:Significant difference between the groups in terms of emotion regulation was observed. The authoritative mothers had the highest score in emotion regulation. Conclusion:Our results indicated that emotion regulation played the main role in different parenting styles.

Objectives:The primary goal of this study was to identify the determinants of mother's authoritative parenting style upon the ecological model of parenting. There are some factors involved in this model such as a parent (i.e. developmental history, personality), and child characteristics (i.e. temperament and developmental issues) and environmental factors. Methods:The statistic population of this study includes mothers in Tehran having preschool children between 4-6 years old. By convenient sampling, eight kindergarten schools were selected, and mothers completed the questionnaires. The sample consisted of 157 mothers who had the authoritative parenting style based on the score of Baumrind's parenting style questionnaire. The participants completed the questionnaires and data was analyzed with regression analysis.Results: The parent's neuroticism (r=-0.253, P<0.01), social support (r=-0.200, P<0.05) and some temperamental characteristics of child i.e. excitability (r=-0.526, P<0.01) and activity (r=-0.163, P<0.05) were significant variables in prediction of authoritative parenting style.Discussion: This study enhances our understanding of the primary determinants of authoritative parenting style in Iranian mothers. The authoritative parenting style is a function of interactional mother and child characteristic and contextual components. These parents had a low score on neuroticism. Therefore, they had emotional stability and could manage their impulse and negative emotions about child maltreatment. Also, their children had low scores in excitability and a high score in sociability. Additionally, the authoritative mothers had weak social support. One explanation for this result is that mothers are the autonomy people and stand on their own rules and had little need to others.

Airway epithelial cells contribute to the first line of mucosal defense by secretion of antimicrobial peptides and antimicrobial lipids, including the cholesteryl ester (CE) cholesteryl arachidonate. Sterol O-acyltransferase 1 (SOAT1) is a key enzyme for CE biosynthesis. However, little is known about the regulation of the lipid-mediated arm of innate host defense. Polarized human bronchial epithelial cells (HBE) were incubated for 6 h with 100 ng/mL LPS, 10 μg/mL peptidoglycan (PG), 25 μg/mL PamCys3, and solvent control. SOAT1 mRNA was quantified by qRT-PCR with duplexing and RPLP0α as housekeeping gene. Lipid content of apical HBE secretions was analyzed by rpHPLC. SOAT1 expression was blocked with siRNA/lipofection. Antibacterial activity of HBE secretions was assessed by a 3 h colony forming unit assay with Pseudomonas aeruginosa (PA). SOAT1 gene expression was significantly increased by LPS (14.6 ±11.4, means ± SD, n=4, p=0.037). Stimulation with PG and PamCys3 increased SOAT1 expression by ~ 4- and 2-fold, respectively. Secretions from LPS-stimulated HBE showed an increase of total lipids including CA by ~30-50%. Selective inhibition of SOAT1 mRNA expression by 80% reduced the antibacterial activity of HBE whereby PA showed enhanced bacterial growth reaching &gt;170% of the controls. These data suggest that SOAT1 is regulated by conserved innate immunity pathways and further support the role of antimicrobial lipids as effector molecules in mucosal airway defense.

Objective: For a long time, it was held that narcissism had two aspects: narcissistic grandiosity and narcissistic fragility. The extraversion, neuroticism, and antagonism elements of the three-factor narcissism paradigm, on the other hand, have gained popularity in recent years. Based on the three-factor framework of narcissism, the Five-Factor Narcissism Inventory-short form (FFNI-SF) is a relatively recent invention. Therefore, this research aimed to assess the validity and reliability of the FFNI-SF in Persian among Iranians. Method: Ten specialists (with Ph.D.s in psychology) were enlisted in this research to translate and evaluate the reliability of the Persian version of the FFNI-SF. The Content Validity Index (CVI) and the Content Validity Ratio (CVR) were then used to assess face and content validity. It was given to 430 students at Azad University, Tehran Medical Branch, once the Persian form was completed. The available sampling technique was used to choose the participants. Cronbach's alpha and the test-retest correlation coefficient were used to assess the reliability of the FFNI-SF. In addition, concept validity was obtained using exploratory factor analysis. In addition, correlations with NEO Five-Factor Inventory (NEO-FFI) and Pathological Narcissism Inventory (PNI) were employed to establish the convergent validity of the FFNI-SF. Results: According to professional opinions, the face and content validity indices met expectations. With Cronbach's alpha and test-retest reliability, the questionnaire's reliability was also established. Cronbach's alphas varied between 0.7 and 0.83 for the FFNI-SF components. According to test-retest reliability coefficients, the components' values varied from 0.7 to 0.86. Additionally, three factors (extraversion, neuroticism, and antagonism) were recovered using the principal components approach and a straight oblimin rotation. According to an analysis of the eigenvalues, the three-factor solution accounted for 49.01 of the variation in the FFNI-SF. The eigenvalues for the three variables were 2.95 (M = 1.39), 2.51 (M = 1.3), and 1.88 (M = 1.24) respectively. The FFNI-SF Persian form's convergent validity was further verified by the association between its results and those from the NEO-FFI and PNI tests and the FFNI-SF. There was a substantial positive association between FFNI-SF Extraversion and NEO Extraversion (r = 0.51, P ≤ 0.001), as well as a strong negative correlation between FFNI-SF antagonism and NEO agreeableness (r = -0.59, P ≤ 0.001). As well as this, PNI grandiose narcissism (r = 0.37, P ≤ 0.001) was shown to be significantly associated with FFNI-SF grandiose narcissism (r = 0.48, P ≤ 0.001), as it was with PNI vulnerable narcissism (r = 0.48, P < 0.001). Conclusion: with its solid psychometric qualities, we may utilize the Persian FFNI-SF to test the three-factor model of narcissism as an effective tool for research.

Acute lymphoblastic leukemia (ALL) is the most common form of childhood cancer. Despite effective chemotherapy, 20% of patients will relapse. Therefore, it is critical that we identify novel therapies to treat ALL. We are studying a new small molecule compound known as tubacin (tubulin acetylation inducer) that selectively inhibits histone deacetylase 6 (HDAC6). HDAC6 binds to polyubiquitinated misfolded proteins and to dynein motor proteins, including alpha-tubulin, thereby recruiting misfolded or unwanted proteins to aggresomes and subsequent degradation by the lysosome. Tubacin was discovered through a chemical genetic screen of a 7,392 small molecule library and was found to induce acetylation of alpha-tubulin by inhibiting one of the two catalytic domains of HDAC6. This inhibition disrupts the interaction of HDAC6 with dynein resulting in marked accumulation of ubiquitinated proteins. Previous work demonstrated that treatment of multiple myeloma cells with tubacin inhibited growth at low micromolar concentrations. Tubacin does not appear to affect global histone acetylation, gene expression, or cell cycle regulation. To determine the effects of tubacin in human ALL cells, we treated both B- and T-cell ALL cell lines with varying concentrations of the drug and performed MTT assays. In T-ALL cells (Jurkat, Loucy), the IC50 of tubacin was found to be 1 to 3 uM, while in B-cell ALL cells (REH, Nalm-6), the IC50 was 2 to 5uM. Tubacin induced apoptosis of Jurkat and Loucy cells stained with Annexin V and propidium iodide. Within 12 hours, we observed increased protein polyubiquitination and PARP cleavage, but no difference in Rb phosphorylation in cells treated with tubacin. Furthermore, tubacin treatment increased acetylation of alpha-tubulin in Loucy and Jurkat cells within 3 hours. To study whether tubacin was toxic to normal hematopoietic cells, we treated human bone marrow cells cultured in methylcellulose containing IL-3, IL-6, and Stem Cell Factor with varying concentrations of tubacin. The IC50 of 20uM was determined from numbers of colonies plated in triplicate. Similarly, treatment of normal human lymphocytes cultured in IL-2, demonstrated an IC50 of 16uM. Finally, we examined the effects of tubacin on growth of primary ALL cells. Bone marrow cells from three patients with B-cell ALL at diagnosis were cultured in tubacin at varying concentrations and MTT assays were performed. In two of the three ALL samples, the IC50 was less than 5uM. Experiments to study the effects of tubacin in mouse models of leukemia are in progress. Our results suggest that inhibition of HDAC6 and the aggresome pathway provides a novel approach to treat ALL.