(1) untreated controls; (2) EIT. Experimental group: (1) EIT + L-NAC (5, 2, or 1 mM); (2) EIT + Mannitol (100, 50, or 10 mM); (3) EIT + Dex (20, 10, or 5 μg/mL); (4) EIT + L-NAC + Mannitol + Dex. After EIT was caused in an in-vitro model of CI, explants were cultured in media containing L-NAC alone, Mannitol alone, or Dex alone at decreasing concentrations. Concentrations of L-NAC, Mannitol, and Dex that showed 50% protection of hair cell loss individually were used as a combination in experimental group 4. Results There was an increase of total hair cell (THC) loss in the EIT OC explants when compared with control group HC counts or the tri-therapy cochlea. This study defined the dosage of L-NAC, Mannitol, and Dex for the survival of 50% protection of hair cells in vitro. Their combination provided close to 96% protection, demonstrating an additive effect.

Medical students have difficulty understanding the mechanisms underlying hyperkalemia-mediated local control of blood flow. Such control mechanisms are crucial in the brain, kidney, and skeletal muscle vasculature. We aimed to identify medical students’ misconceptions via assessment of students’ in-class knowledge and, subsequently, improve future teaching of this concept. In-class polling was performed with the TurningPoint clicker response system ( n = 860) to gauge students’ understanding of three physiological concepts related to hyperkalemia: membrane potential ( Vm), conductance, and smooth muscle response. Vm includes the concepts of equilibrium potential ( Veq) for specific ions, as well as driving force (DF =  Vm − Veq). Students understood the concept of DF (~70% answered correctly), suggesting their understanding of Vm. However, students misunderstood that hyperkalemia results in depolarization (~52% answered correctly) and leads to an increase in potassium conductance (~31% answered correctly). Clarification of the type of smooth muscle as vascular increased the percentage of correct responses (~51 to 73%). The data indicate that students lacked knowledge of specific potassium conductance in various muscle types, resulting in divergent responses, such as the canonical depolarization in skeletal muscle versus hyperpolarization in smooth muscle cells during hyperkalemia. Misunderstanding of this crucial concept of conductance is directly related to the students’ performance. Furthermore, we connected the paradoxical effect of hyperkalemia to pathological acute and chronic hyperkalemia clinical scenarios.

Table 1. (continued) Appropriateness of Order Median time to colonoscopy, days (IQR) 53 (27-95) Adequate bowel preparation (%) 47 (84) Documentation of cecal intubation (%) 55 (98) Documentation of appendiceal orifice and IC valve visualization (%) 48 (94) Documentation of right colon retroflexion (%) 44 (83) Advanced adenoma found on colonoscopy (%) 16 (29) Other adenoma or sessile polyp found on colonoscopy (%) 19 (34) Negative (%) 409 (85) Documentation of a 3-year follow-up screening recommendation (%) 369 (80) S297

No abstract