Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.

The light-dependent magnetic compass sense of night-migratory songbirds can be disrupted by weak radiofrequency fields. This finding supports a quantum mechanical, radical-pair-based mechanism of magnetoreception as observed for isolated cryptochrome 4, a protein found in birds’ retinas. The exact identity of the magnetically sensitive radicals in cryptochrome is uncertain in vivo, but their formation seems to require a bound flavin adenine dinucleotide chromophore and a chain of four tryptophan residues within the protein. Resulting from the hyperfine interactions of nuclear spins with the unpaired electrons, the sensitivity of the radicals to radiofrequency magnetic fields depends strongly on the number of magnetic nuclei (hydrogen and nitrogen atoms) they contain. Quantum-chemical calculations suggested that electromagnetic noise in the frequency range 75–85 MHz could give information about the identity of the radicals involved. Here, we show that broadband 75–85 MHz radiofrequency fields prevent a night-migratory songbird from using its magnetic compass in behavioural experiments. These results indicate that at least one of the components of the radical pair involved in the sensory process of avian magnetoreception must contain a substantial number of strong hyperfine interactions as would be the case if a flavin–tryptophan radical pair were the magnetic sensor.

Background: Night-migratory birds sense the Earth´s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that light-induced formation of a radical-pair in European robin cryptochrome 4a, ErCry4a, is the primary signalling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signalling involving the α-subunit of the cone specific heterotrimeric G protein from European robin. Methods: Protein-protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in vivo and might constitute the first biochemical signalling step in radical-pair-based magnetoreception.

Every year, billions of animals leave their home range and start seasonal migrations in order to find more favorable resources and to escape harsh environmental conditions. These round trips often span thousands of kilometers. To successfully navigate along their route, animals rely on various external references. While landmarks and celestial cues like stars or the sun are easy to imagine as guidance on these journeys, using the geomagnetic field for orientation is more elusive. The geomagnetic field is an omnipresent cue, which can be sensed and relied upon by many animals, even when visual cues are sparse. How magnetic fields can be perceived seems to vary between birds and fish. While birds seem to use a mechanism based on the quantum mechanical properties of electron spins, fish may have evolved a compass similar in its function to the technical devises developed by humans. How these mechanisms work precisely and how they are integrated are research questions addressed in SFB 1372.

The role of inherited orientation programmes in determining the outbound migratory routes of birds is increasingly well understood, though less is known about the influence of inherited information on return migration. Previous studies suggest that spatial gradient cues learnt through experience could be of considerable importance when relocating the natal site, though such cues could, in principle, augment rather than replace inherited migratory information. Here, we show that juvenile Eurasian blackcaps ( Sylvia atricapilla ) that have never left northwest Europe (i.e. never had the opportunity to learn navigational information on a continental scale) show significant spring orientation in a direction near-identical to that expected based on ringing recoveries from free-flying individuals. We suggest that this is probably indicative of birds inheriting an orientation programme for spring as well as autumn migration and speculate that, as long as the birds are not displaced far from their normal migration route, the use of inherited spring migratory trajectories might make uni-coordinate ‘stop signs’ sufficiently accurate for the long-distance targeting of their breeding sites.