outcomes, we performed a 10-week biochemistry Rain Classroom teaching experiment among students majoring inclinical medicine. Rain Classroom is a plug-in for WeChat, a smartphone instant messaging application. Teachers canpost teaching resources on the Rain Classroom platform, administer tests, and communicate with students. RainClassroom can also be used to automatically collect course learning data from students. When teaching is complete,questionnaire surveys can be conducted, and test scores can be outputted. Results showed that students wereinterested in the biochemistry Rain Classroom teaching application, and that the application increased theirenthusiasm for the course materials. There were significant improvements in the learning outcomes in students givenRain Classroom teaching, compared to those given traditional PowerPoint slideshow lessons. We conclude that theRain Classroom tool is a new mobile learning application that promotes self-learning and improves learningoutcomes in biochemistry learning.