Sex pheromones are commonly used in traps to monitor populations and movements of male click beetles, but to date few attractants have been identified for females. Notable exceptions are plant-derived kairomones for female Agriotes brevis and A. ustulatus, allowing the monitoring of both males and females of these species with lures containing both pheromones and plant volatiles. The attractiveness of these plant volatiles for two congeners, A. obscurus and A. lineatus, which are agricultural pests in Europe and North America, was evaluated in the current study. Both the four-component MINIM plant-derived lure for A. brevis, and the blend of (E)-anethol and (E)-cinnamaldehyde for A. ustulatus, were not attractive to A. obscurus and A. lineatus, and instead appeared to reduce captures—both when compared to blank controls, and when blended with and compared to the sex pheromones of these species. This was most pronounced in A. obscurus, where (E)-anethol and (E)-cinnamaldehyde reduced male captures by 43 and 37%, respectively. Combining the pheromones of A. obscurus and A. lineatus reduced captures of these species by 77 and 19%, respectively, compared to these pheromones singly. This suggests that attractants for female click beetles can be highly species-specific, and that the blending of pheromones of congeneric species with each other, or with plant volatiles, can reduce captures. Further research into developing such attractants for economic species is urgently needed.
Field collected wireworms (Coleoptera: Elateridae) intended for laboratory studies often die during storage from Metarhizium Sorokin (Clavicipitaceae) infection and other causes. To determine optimal storage conditions for Agriotes obscurus (Linnaeus), freshly collected larvae were stored in regular and sterilised soil, with and without access to food (potato slices), at 12 °C and 16 °C, for six months to determine which conditions resulted in the lowest wireworm mortality. Survival was highest when wireworms were stored in sterilised soil, at 12 °C, in the absence of food. Food availability increased wireworm mortality from Metarhizium infection and other causes, but decreased the number of missing wireworms. Wireworm weight change during the study was highest in treatments where wireworms had access to food. The expected effect of food deprivation on wireworm energetic state and immune response are discussed in context with these findings.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.