Staphylococcus aureus is a leading cause of nosocomial and community-associated infection worldwide; however, there is no licensed vaccine available. S. aureus initiates infection via the mucosa; therefore, a mucosal vaccine is likely to be a promising approach against S. aureus infection. Lactobacilli, a non-pathogenic bacterium, has gained increasing interest as a mucosal delivery vehicle. Hence, we attempted to develop an oral S. aureus vaccine based on lactobacilli to cushion the stress of drug resistance and vaccine needs. In this study, we designed, constructed, and evaluated recombinant Lactobacillus strains synthesizing S. aureus nontoxic mutated α-hemolysins (HlaH35L). The results from animal clinical trials showed that recombinant Lactobacillus can persist for at least 72 h and can stably express heterologous protein in vivo. Recombinant L. plantarum WXD234 (pNZ8148-Hla) could induce robust mucosal immunity in the GALT, as evidenced by a significant increase in IgA and IL-17 production and the strong proliferation of T-lymphocytes derived from Peyer’s patches. WXD234 (pNZ8148-Hla) conferred up to 83% protection against S. aureus pulmonary infection and significantly reduced the abscess size in a S. aureus skin infection model. Of particular interest is the sharp reduction of the protective effect offered by WXD234 (pNZ8148-Hla) vaccination in γδ T cell-deficient or IL-17-deficient mice. In conclusion, for the first time, genetically engineered Lactobacillus WXD234 (pNZ8148-Hla) as an oral vaccine induced superior mucosal immunity, which was associated with high protection against pulmonary and skin infections caused by S. aureus. Taken together, our findings suggest the great potential for a delivery system based on lactobacilli and provide experimental data for the development of mucosal vaccines for S. aureus.

Probiotics are gaining attention due to their functions of regulating the intestinal barrier and promoting human health. The production of exopolysaccharide (EPS) is one of the important factors for probiotics to exert beneficial properties. This study aimed to screen exopolysaccharides-producing lactic acid bacteria (LAB) and evaluate the probiotic potential. we obtained three exopolysaccharide fractions (EPS1, EPS2, and EPS3) from Lactobacillus pantheris TCP102 and purified by a combination of ion-exchange chromatography and gel permeation chromatography. The structures of the fractions were characterized by FT-IR, UV, HPLC, and scanning electron microscopy (SEM) analysis. The Mw of EPS1, EPS2, and EPS3 were approximately 20.3, 23.0, and 19.3 kDa, and were mainly composed of galactose, glucose, and mannose, with approximate molar ratios of 2.86:1:1.48, 1.26:1:1, 1.58:1.80:1, respectively. Furthermore, SEM analysis demonstrated that the three polysaccharide fractions differ in microstructure and surface morphology. Additionally, preliminary results for immune-enhancing and anticancer activities reveal that these EPSs significantly induced the production of nitric oxide (NO), TNF-α, and IL-6 in Ana-1 cells and peritoneal macrophage cells. Meanwhile, the EPSs also significantly suppressed the proliferation of HCT-116, BCG-803, and particularly A-2780 cells. The results suggest that the three novel EPSs isolated from Lactobacillus pantheris TCP102 can be regarded as potential application value in functional food and natural antitumor drugs.

We systematically screened and evaluated protective effects of engineered Lactobacillus against S. aureus infection in terms of differing delivery vehicle strains and S. aureus antigens and in localized and systemic infection models. Engineered L. reuteri was developed and showed strong protective effects against both types of S. aureus -induced infection.

The neritic region of the Chaoshan plain is located on the northeastern (NE) boundary of the South China Sea (SCS). Despite the extensive research on the stratigraphic architecture and sedimentary processes within the Chaoshan plain, the neighboring neritic area remains largely unexplored. In this study, we provide a new set of ages on seventeen quartz optically stimulated luminescence (OSL) dating results from four cores (SY2-2, SY3-1, SY3-2, and HS02) to investigate the regional chronostratigraphy and sedimentology of the area. The samples were collected at depths ranging from 0.6 to 73.5 m and yielded ages ranging from 4.9 ± 0.3 ka to >139 ± 28 ka. Sedimentation thickness from cores SY3-2, SY2-2, and HS02 is less than 4.2 m, 5.5 m, and 6.4 m, respectively, since at least Marine Isotope Stage (MIS) 5, and the Holocene sediments of core SY3-2 are less than 4.2 m thick. The preservation state of the sediments in the area is poor since at least 83.6 ka, indicating an intensified erosion in the neritic region of the northeastern South China Sea (NESCS) since at least MIS 5. This erosion may have been caused by fluvial incision resulting from sea-level decline during the last glacial period, as well as strong transportation caused by coastal currents.

With the continuous improvement of the national social and economic level the size and quantity of various types of infrastructure in our country have been improved and the community has put forward higher requirements on the level and quality of the construction. In recent years the bridge collapse accidents occur frequently, but our country bridge inspection and safety assessment of the system was established late, our country bridge structure health monitoring system research and application began in the 90. of the 20th century, the implementation of large-scale infrastructure construction projects, bridge health monitoring system also will be rapid development this paper discusses the based on BIM bridge inspection and safety assessment system, and puts forward some practical measures, provides the reference for improving bridge inspection and safety assessment of the system operation level.