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In a process of insect footprint recognition, footprint segments need to be extracted from scanned insect footprints in order to find out appropriate features for classification. In this paper, we use a clustering method in a preprocessing stage for extraction of insect footprint segments. In general, sizes and strides of footprints may be different according to type and size of an insect for recognition. Therefore we propose a method for insect footprint segment extraction using an improved ART2 algorithm regardless of size and stride of footprint pattern. In the improved ART2 algorithm, an initial threshold value for clustering is determined automatically using the contour shape of the graph created by accumulating distances between all the spots within a binarized footprint pattern image. In the experimental results, applying the proposed method to two kinds of insect footprint patterns, we illustrate that clustering is accomplished correctly.