This study was conducted to investigate the incidence of rice stem borer infestations and the species composition present in the selected rice fields. Data were collected between April 2017 and November 2018 across three locations, which include the upland rice field in Agbajege, Ogun state, rainfed lowland in Itoikin and Mangrove swamp in Igbogun, Lagos state, respectively. The incidence of rice stem borers was indicated by dead heart and white heads symptoms, while tillers were excised for rice stem borer identification and population. Results showed a high and low incidence of rice stem borers with no significant difference(p>0.05) at p= 0.29 and 0.37 between the early and late planting seasons in the rainfed lowland and upland rice fields respectively. Planting seasons were significant in the incidence of rice stem borers in mangrove swamp rice fields at p<0.05, p=0.01). This study showed that the predominant species of rice stem borers present in the three study sites were Chilo zacconius and Sesamia calamistis. C. zacconius was the most abundant species at 89% while S. calamistis was 11%. Rice stem borer populations were significantly higher at (p<0.05, p=0.000) in flooded zones while the highest rice stem borer populations were observed at the reproductive phase of the rice plants. Thisstudy showed that the planting seasons coincided with the peak period of the development of rice stem borers. Hence, conservation of natural enemies, monitoring and surveillance should be adopted in insect pest management in rice agroecosystems