Mycobacterium massiliense 33 MAM. 42.9% MAB complex isolates were MDR. Macrolide resistant MAB and 34 MAM had T28 sequevar, showing functional erm(41) responsible for inducible 35 resistance. Unexpectedly, full length erm(41) was found in MAM. Therrl gene in these 36 isolates showed no point mutations, indicating T28 sequevar as cause of inducible 37 resistance. All fluoroquinolone resistant isolates showed Ala-83 in gyrA 38 fluoroquinolone resistant-dependent region (QRDR) and Arg-447 and Asn-464 39 in gyrB QRDR. These are associated with resistance to the drug. In the Philippines, one of the most common rapidly growing mycobacteria (RGM) from 43 patients with multiple drug resistant tuberculosis (MDR-TB) infection is M. abscessus 44 complex. This non-tuberculous Mycobacterium (NTM) species is usually found in TB 45 culture, either growing alongside with M. tuberculosis or after the patient has been 46 confirmed negative for pulmonary tuberculosis through direct sputum smear 47 microscopy (DSSM) and TB culture. Pulmonary diseases caused by the M. abscessus 48 complex are extremely hard to manage for these species are found to be resistant to 49 anti-tuberculous agents. Patients with M. abscessus infection do not receive appropriate 50 treatment for they are considered to have chronic TB and MDR-TB. As a result, 51 patients are prescribed a six-month anti-TB treatment which is not appropriate for M. 52 abscessus infections. Interestingly, there is no known study in the Philippines regarding 53 the antimicrobial resistance profile of M. abscessus complex. NTM studies are 54 overshadowed by pulmonary TB research studies and therefore, clinicians are not 55 guided on the treatment for NTM infections. Moreover, detection of NTM species 56 is not part of the routine procedure in the clinical microbiology laboratory. Treatment 57 failure to both first-line anti-TB drugs rifampicin and isoniazid is linked to infection 58 with multidrug-resistant M. tuberculosis (MDR-TB). Gler et al. (2012) showed very 59 high rates (83% to 97%) of MDR-TB infections in the Philippines in 2012. Because 60 MDR TB is often associated with the occurrence of M. abscessus complex in clinical 61 samples, the present study aimed to characterize local isolates of M. abscessus complex 62 from patients with multidrug-resistant pulmonary tuberculosis in terms of their 63 antimicrobial susceptibility profiles and determine the genetics of the antibiotic 101 Center of the Philippines from which these 20 isolates identified to be M. abscessus complex were taken. 102 103 Phenotypic characterization of Mycobacterium abscessus complex 104 Freshly grown 5 to 7-day old cultures of M. abscessus complex were subjected to 105 phenotypic characterization. Colony morphology was determined by observing the 106 colonies grown on Ogawa medium. There are two possible morphotypes of M.